Single-cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome

P Paganos, D Voronov, JM Musser, D Arendt… - Elife, 2021 - elifesciences.org
Identifying the molecular fingerprint of organismal cell types is key for understanding their
function and evolution. Here, we use single-cell RNA sequencing (scRNA-seq) to survey the …

[HTML][HTML] Plastic leachate-induced toxicity during sea urchin embryonic development: Insights into the molecular pathways affected by PVC

P Paganos, CV Ullmann, D Gaglio, M Bonanomi… - Science of The Total …, 2023 - Elsevier
Microplastics are now polluting all seas and, while studies have found numerous negative
interactions between plastic pollution and marine animals, the effects on embryonic …

Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ

M Perillo, SZ Swartz, C Pieplow, GM Wessel - Nature Communications, 2023 - nature.com
A fundamental goal in the organogenesis field is to understand how cells organize into
tubular shapes. Toward this aim, we have established the hydro-vascular organ in the sea …

Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins

P Paganos, P Ronchi, J Carl, G Mizzon… - Frontiers in cell and …, 2022 - frontiersin.org
The identity and function of a given cell type relies on the differential expression of gene
batteries that promote diverse phenotypes and functional specificities. Therefore, the …

A New Model Organism to Investigate Extraocular Photoreception: Opsin and Retinal Gene Expression in the Sea Urchin Paracentrotus lividus

P Paganos, E Ullrich-Lüter, F Caccavale, A Zakrzewski… - Cells, 2022 - mdpi.com
Molecular research on the evolution of extraocular photoreception has drawn attention to
photosensitive animals lacking proper eye organs. Outside of vertebrates, little is known …

Characterization of thyrotropin-releasing hormone producing neurons in sea urchin, from larva to juvenile

M Cocurullo, P Paganos, G Benvenuto… - Frontiers in …, 2024 - frontiersin.org
Most sea urchin species are indirect developers, going through a larval stage called pluteus.
The pluteus possesses its own nervous system, consisting mainly of the apical organ …

Single-cell transcriptomic analysis reveals the molecular profile of go-opsin photoreceptor cells in sea urchin larvae

M Cocurullo, P Paganos, R Annunziata, D Voronov… - Cells, 2023 - mdpi.com
The ability to perceive and respond to light stimuli is fundamental not only for spatial vision
but also to many other light-mediated interactions with the environment. In animals, light …

FISH for All: A Fast and Efficient Fluorescent In situ Hybridization (FISH) Protocol for Marine Embryos and Larvae

P Paganos, F Caccavale, C La Vecchia… - Frontiers in …, 2022 - frontiersin.org
In situ hybridization is one the most commonly used techniques for developmental and
evolutionary biology and has extensively contributed to the identification of distinct cell types …

[HTML][HTML] Gene regulatory divergence amongst echinoderms underlies appearance of pigment cells in sea urchin development

M Spurrell, N Oulhen, S Foster, M Perillo… - Developmental …, 2023 - Elsevier
Larvae of the sea urchin, Strongylocentrotus purpuratus, have pigmented migratory cells
implicated in immune defense and gut patterning. The transcription factor SpGcm activates …

Molecular and Cellular Characterization of the TH Pathway in the Sea Urchin Strongylocentrotus purpuratus

M Cocurullo, P Paganos, NJ Wood, MI Arnone… - Cells, 2023 - mdpi.com
Thyroid Hormones (THs) are a class of signaling molecules produced by coupling iodine
with tyrosine residues. In vertebrates, extensive data support their important role in a variety …