Mass spectrometry–based proteomics turns quantitative

SE Ong, M Mann - Nature chemical biology, 2005 - nature.com
The field of proteomics is built on technologies to analyze large numbers of proteins—ideally
the entire proteome—in the same experiment. Mass spectrometry (MS) has been …

Proteomics by mass spectrometry: approaches, advances, and applications

JR Yates, CI Ruse… - Annual review of …, 2009 - annualreviews.org
Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis

JV Olsen, M Vermeulen, A Santamaria, C Kumar… - Science …, 2010 - science.org
Eukaryotic cells replicate by a complex series of evolutionarily conserved events that are
tightly regulated at defined stages of the cell division cycle. Progression through this cycle …

Quantitative, high-resolution proteomics for data-driven systems biology

J Cox, M Mann - Annual review of biochemistry, 2011 - annualreviews.org
Systems biology requires comprehensive data at all molecular levels. Mass spectrometry
(MS)-based proteomics has emerged as a powerful and universal method for the global …

Quantitative proteomic analysis of histone modifications

H Huang, S Lin, BA Garcia, Y Zhao - Chemical reviews, 2015 - ACS Publications
Several mechanisms have been proposed for histone marks to exert their functions,
including altering the physical properties of nucleosomes by neutralization of charge via …

Proteomics: a pragmatic perspective

P Mallick, B Kuster - Nature biotechnology, 2010 - nature.com
The evolution of mass spectrometry–based proteomic technologies has advanced our
understanding of the complex and dynamic nature of proteomes while concurrently …

The lysin motif receptor-like kinase (LysM-RLK) CERK1 is a major chitin-binding protein in Arabidopsis thaliana and subject to chitin-induced phosphorylation*♦

EK Petutschnig, AME Jones, L Serazetdinova… - Journal of Biological …, 2010 - ASBMB
Plants detect potential pathogens by sensing microbe-associated molecular patterns via
pattern recognition receptors. In the dicot model plant Arabidopsis, the lysin motif (LysM) …

Interpreting the protein language using proteomics

ON Jensen - Nature reviews Molecular cell biology, 2006 - nature.com
Post-translational modifications define the functional and structural plasticity of proteins in
archaea, prokaryotes and eukaryotes. Multi-site protein modification modulates protein …

Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue

R Martello, M Leutert, S Jungmichel, V Bilan… - Nature …, 2016 - nature.com
Although protein ADP-ribosylation is involved in diverse biological processes, it has
remained a challenge to identify ADP-ribose acceptor sites. Here, we present an …

Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level

JMR Danielsen, KB Sylvestersen… - Molecular & Cellular …, 2011 - ASBMB
The covalent attachment of ubiquitin to proteins regulates numerous processes in eukaryotic
cells. Here we report the identification of 753 unique lysine ubiquitylation sites on 471 …