Molecular photoswitches enable dynamic control of processes with high spatiotemporal
precision, using light as external stimulus, and hence are ideal tools for different research …

In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

The quality and application of super-resolution fluorescence imaging greatly lie in the dyes'
properties, including photostability, brightness, and Stokes shift. Here we report a synergistic …

Stimulated emission depletion (STED) nanoscopy is a promising fluorescence microscopy to
detect unresolvable structures at the nanoscale level and then achieve a superior imaging …

Rhodamine dyes exist in equilibrium between a fluorescent zwitterion and a nonfluorescent
lactone. Tuning this equilibrium toward the nonfluorescent lactone form can improve cell …

Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as
protein tags for multicolor microscopy and in vivo imaging. Here we apply rational design to …

The development of live-cell fluorescence nanoscopy is powered by the availability of
suitable fluorescent probes. Rhodamines are among the best fluorophores for labeling …

For many years, different probing techniques have mainly relied on antibodies for molecular
recognition. However, with the discovery of aptamers, this has changed. The science …