DNA metabarcoding in diet studies: Unveiling ecological aspects in aquatic and terrestrial ecosystems

LL de Sousa, SM Silva, R Xavier - Environmental DNA, 2019 - Wiley Online Library
Effective conservation of species and ecosystems requires the understanding of important
ecological traits, such as dietary habits, food webs, and trophic niches. In diet studies, the …

Soil nematode abundance and functional group composition at a global scale

J Van Den Hoogen, S Geisen, D Routh, H Ferris… - Nature, 2019 - nature.com
Soil organisms are a crucial part of the terrestrial biosphere. Despite their importance for
ecosystem functioning, few quantitative, spatially explicit models of the active belowground …

High‐throughput identification and diagnostics of pathogens and pests: overview and practical recommendations

L Tedersoo, R Drenkhan, S Anslan… - Molecular ecology …, 2019 - Wiley Online Library
High‐throughput identification technologies provide efficient tools for understanding the
ecology and functioning of microorganisms. Yet, these methods have been only rarely used …

Exploring the gastrointestinal “nemabiome”: deep amplicon sequencing to quantify the species composition of parasitic nematode communities

RW Avramenko, EM Redman, R Lewis, TA Yazwinski… - PLoS …, 2015 - journals.plos.org
Parasitic helminth infections have a considerable impact on global human health as well as
animal welfare and production. Although co-infection with multiple parasite species within a …

Freshwater monitoring by nanopore sequencing

L Urban, A Holzer, JJ Baronas, MB Hall… - Elife, 2021 - elifesciences.org
While traditional microbiological freshwater tests focus on the detection of specific bacterial
indicator species, including pathogens, direct tracing of all aquatic DNA through …

Characterization of soil nematode communities in three crop** systems through morphological and DNA metabarcoding approaches

AM Treonis, SK Unangst, RM Kepler, JS Buyer… - Scientific reports, 2018 - nature.com
We used complementary morphological and DNA metabarcoding approaches to
characterize soil nematode communities in three crop** systems, conventional till (CT), no …

Integrating quantitative morphological and qualitative molecular methods to analyse soil nematode community responses to plant range expansion

S Geisen, LB Snoek, FC ten Hooven… - Methods in Ecology …, 2018 - Wiley Online Library
Below‐ground nematodes are important for soil functioning, as they are ubiquitous and
operate at various trophic levels in the soil food web. However, morphological nematode …

[HTML][HTML] Nematodes as bioindicators of polluted sediments using metabarcoding and microscopic taxonomy

J Schenk, S Höss, M Brinke, N Kleinbölting… - Environment …, 2020 - Elsevier
The use of bioindicator species is a widely applied approach to evaluate ecological
conditions, and several indices have been designed for this purpose. To assess the impact …

Using high-throughput sequencing quantitatively to investigate soil nematode community composition in a steppe-forest ecotone

XF Du, YB Li, X Han, W Ahmad, Q Li - Applied Soil Ecology, 2020 - Elsevier
The analysis of nematode community composition by high throughput sequencing (HTS)
has attracted much attention because of its advantage for throughput, high resolution and …

Improving accuracy of DNA diet estimates using food tissue control materials and an evaluation of proxies for digestion bias

AC Thomas, SN Jarman, KH Haman… - Molecular …, 2014 - Wiley Online Library
Ecologists are increasingly interested in quantifying consumer diets based on food DNA in
dietary samples and high‐throughput sequencing of marker genes. It is tempting to assume …