Towards higher sensitivity of mass spectrometry: A perspective from the mass analyzers

C Li, S Chu, S Tan, X Yin, Y Jiang, X Dai… - Frontiers in …, 2021 - frontiersin.org
Mass spectrometry (MS) is one of the most widely used analytical techniques in many fields.
Recent developments in chemical and biological researches have drawn much attention to …

Challenges and emergent solutions for LC‐MS/MS based untargeted metabolomics in diseases

L Cui, H Lu, YH Lee - Mass spectrometry reviews, 2018 - Wiley Online Library
In the past decade, advances in liquid chromatography‐mass spectrometry (LC‐MS) have
revolutionized untargeted metabolomics analyses. By mining metabolomes more deeply …

Parallelized acquisition of orbitrap and astral analyzers enables high-throughput quantitative analysis

HI Stewart, D Grinfeld, A Giannakopulos… - Analytical …, 2023 - ACS Publications
The growing trend toward high-throughput proteomics demands rapid liquid
chromatography–mass spectrometry (LC–MS) cycles that limit the available time to gather …

[HTML][HTML] A compact quadrupole-orbitrap mass spectrometer with FAIMS interface improves proteome coverage in short LC gradients

DB Bekker-Jensen, A Martínez-Val… - Molecular & Cellular …, 2020 - Elsevier
State-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and
their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per …

Optimal Dissociation Methods Differ for N- and O-Glycopeptides

NM Riley, SA Malaker, MD Driessen… - Journal of proteome …, 2020 - ACS Publications
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and
recent efforts have focused on how to best interrogate glycopeptides using tandem mass …

Progress in top-down proteomics and the analysis of proteoforms

TK Toby, L Fornelli, NL Kelleher - Annual review of analytical …, 2016 - annualreviews.org
From a molecular perspective, enactors of function in biology are intact proteins that can be
variably modified at the genetic, transcriptional, or post-translational level. Over the past 30 …

A novel mechanism of host-pathogen interaction through sRNA in bacterial outer membrane vesicles

K Koeppen, TH Hampton, M Jarek, M Scharfe… - PLoS …, 2016 - journals.plos.org
Bacterial outer membrane vesicle (OMV)-mediated delivery of proteins to host cells is an
important mechanism of host-pathogen communication. Emerging evidence suggests that …

Cross-linking mass spectrometry (XL-MS): an emerging technology for interactomics and structural biology

C Yu, L Huang - Analytical chemistry, 2017 - pmc.ncbi.nlm.nih.gov
Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …

Benchmarking common quantification strategies for large-scale phosphoproteomics

A Hogrebe, L von Stechow, DB Bekker-Jensen… - Nature …, 2018 - nature.com
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but
reproducible quantification remains challenging, especially for post-translational …

[HTML][HTML] Extended multiplexing of tandem mass tags (TMT) labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue

DL Plubell, PA Wilmarth, Y Zhao, AM Fenton… - Molecular & Cellular …, 2017 - Elsevier
The lack of high-throughput methods to analyze the adipose tissue protein composition
limits our understanding of the protein networks responsible for age and diet related …