Cre recombinase

GD Van Duyne - Mobile DNA III, 2015 - Wiley Online Library
The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

An overview of tyrosine site‐specific recombination: From an FLP perspective

M Jayaram, CH Ma, AH Kachroo, PA Rowley… - Mobile DNA …, 2015 - Wiley Online Library
Tyrosine family site‐specific recombinases (YRs), named after the active site tyrosine
nucleophile they utilize for DNA strand breakage, are widely distributed among prokaryotes …

DNA origami based visualization system for studying site-specific recombination events

Y Suzuki, M Endo, Y Katsuda, K Ou… - Journal of the …, 2014 - ACS Publications
Site-specific recombination involves reciprocal exchange between defined DNA sites. The
reaction initiates from the formation of a recombinase–DNA synaptic complex, in which two …

Biomolecular topology: Modelling and analysis

J Liu, KL **a, J Wu, SST Yau, GW Wei - Acta Mathematica Sinica, English …, 2022 - Springer
With the great advancement of experimental tools, a tremendous amount of biomolecular
data has been generated and accumulated in various databases. The high dimensionality …

Visualization of DNA and protein–DNA complexes with atomic force microscopy

YL Lyubchenko, AA Gall, LS Shlyakhtenko - Electron Microscopy: Methods …, 2014 - Springer
This article describes sample preparation techniques for AFM imaging of DNA and protein–
DNA complexes. The approach is based on chemical functionalization of the mica surface …

Synapsis of loxP sites by Cre recombinase

K Ghosh, F Guo, GD Van Duyne - Journal of Biological Chemistry, 2007 - jbc.org
Cre recombinase catalyzes site-specific recombination between 34-bp loxP sites in a variety
of topological and cellular contexts. An obligatory step in the recombination reaction is the …

Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence

JNM Pinkney, P Zawadzki, J Mazuryk… - Proceedings of the …, 2012 - pnas.org
Site-specific recombination plays key roles in microbe biology and is exploited extensively to
manipulate the genomes of higher organisms. Cre is a well studied site-specific …

FliZ Acts as a Repressor of the ydiV Gene, Which Encodes an Anti-FlhD4C2 Factor of the Flagellar Regulon in Salmonella enterica Serovar Typhimurium

T Wada, Y Tanabe, K Kutsukake - Journal of bacteriology, 2011 - journals.asm.org
YdiV acts as an anti-FlhD4C2 factor, which negatively regulates the class 2 flagellar operons
in poor medium in Salmonella enterica serovar Typhimurium. On the other hand, one of the …

Fractionation of SWNT/nucleic acid complexes by agarose gel electrophoresis

AA Vetcher, S Srinivasan, IA Vetcher… - …, 2006 - iopscience.iop.org
We show that aqueous dispersions of single-walled carbon nanotubes (SWNTs), prepared
with the aid of nucleic acids (NAs) such as RNA or DNA, can be separated into fractions …

Assembly of Synaptic Protein–DNA Complexes: Critical Role of Non-Specific Interactions

S Vemulapalli, M Hashemi, AB Kolomeisky… - International journal of …, 2023 - mdpi.com
The synaptic protein–DNA complexes, formed by specialized proteins that bridge two or
more distant sites on DNA, are critically involved in various genetic processes. However, the …