Hyperoxia causes mitochondrial fragmentation in pulmonary endothelial cells by increasing expression of pro-fission proteins

C Ma, AM Beyer, M Durand, AV Clough… - … , and vascular biology, 2018‏ - ahajournals.org
Objective—We explored mechanisms that alter mitochondrial structure and function in
pulmonary endothelial cells (PEC) function after hyperoxia. Approach and Results …

Quantification of mitochondrial membrane potential in the isolated rat lung using rhodamine 6G

SH Audi, A Cammarata, AV Clough… - Journal of Applied …, 2020‏ - journals.physiology.org
Mitochondrial membrane potential (Δψm) plays a key role in vital mitochondrial functions,
and its dissipation is a hallmark of mitochondrial dysfunction. The objective of this study was …

Assessment of protection offered by the NRF2 pathway against hyperoxia-induced acute lung injury in NRF2 knockout rats

SH Audi, ER Jacobs, P Taheri, S Ganesh, AV Clough - Shock, 2022‏ - journals.lww.com
Nuclear factor erythroid 2-related factor (Nrf2) is a redox-sensitive transcription factor that
responds to oxidative stress by activating expressions of key antioxidant and cytoprotective …

Protection by inhaled hydrogen therapy in a rat model of acute lung injury can be tracked in vivo using molecular imaging

SH Audi, ER Jacobs, X Zhang, AKS Camara, M Zhao… - Shock, 2017‏ - journals.lww.com
Inhaled hydrogen gas (H 2) provides protection in rat models of human acute lung injury
(ALI). We previously reported that biomarker imaging can detect oxidative stress and …

Detection of hydrogen peroxide production in the isolated rat lung using Amplex red

SH Audi, N Friedly, RK Dash, AM Beyer… - Free radical …, 2018‏ - Taylor & Francis
The objectives of this study were to develop a robust protocol to measure the rate of
hydrogen peroxide (H2O2) production in isolated perfused rat lungs, as an index of …

Integrated computational model of the bioenergetics of isolated lung mitochondria

X Zhang, RK Dash, ER Jacobs, AKS Camara… - PLoS …, 2018‏ - journals.plos.org
Integrated computational modeling provides a mechanistic and quantitative framework for
describing lung mitochondrial bioenergetics. Thus, the objective of this study was to develop …

99MTc-hexamethylpropyleneamine oxime imaging for early detection of acute lung injury in rats exposed to hyperoxia or lipopolysaccharide treatment

SH Audi, AV Clough, ST Haworth, M Medhora, M Ranji… - Shock, 2016‏ - journals.lww.com
ABSTRACT 99m Tc-Hexamethylpropyleneamine oxime (HMPAO) is a clinical single-photon
emission computed tomography biomarker of tissue oxidoreductive state. Our objective was …

Time‐lapse microscopy of oxidative stress demonstrates metabolic sensitivity of retinal pericytes under high glucose condition

Z Ghanian, S Mehrvar, N Jamali… - Journal of …, 2018‏ - Wiley Online Library
Hyperglycemia affects retinal vascular cell function, promotes the development and
progression of diabetic retinopathy and ultimately causes vision loss. Oxidative stress …

Depolarized mitochondrial membrane potential and protection with duroquinone in isolated perfused lungs from rats exposed to hyperoxia

SH Audi, S Ganesh, P Taheri… - Journal of Applied …, 2022‏ - journals.physiology.org
Dissipation of mitochondrial membrane potential (Δψm) is a hallmark of mitochondrial
dysfunction. Our objective was to use a previously developed experimental-computational …

In vivo molecular imaging stratifies rats with different susceptibilities to hyperoxic acute lung injury

SH Audi, P Taheri, M Zhao, K Hu… - … of Physiology-Lung …, 2022‏ - journals.physiology.org
99mTc-hexamethylpropyleneamine oxime (HMPAO) and 99mTc-duramycin in vivo imaging
detects pulmonary oxidative stress and cell death, respectively, in rats exposed to> 95% O2 …