Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …
DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA
S Li, H Yin, J Zheng, Y Wan, K Wang, C Yang… - ACS …, 2024 - ACS Publications
Rapid and precise nucleic acid testing at the point-of-care (POC) is essential for effective
screening and management of infectious diseases. Current polymerase-based molecular …
screening and management of infectious diseases. Current polymerase-based molecular …
Microfluidic encapsulation of DNAs in liquid beads for digital PCR application
Droplet-based microfluidics and digital polymerase chain reaction (PCR) hold significant
promise for accurately detecting and quantifying pathogens. However, existing droplet …
promise for accurately detecting and quantifying pathogens. However, existing droplet …
Hydrogel-Based Microdroplet Ensembles Encapsulating Multiplexed EXPAR Assays for Trichromic Digital Profiling of MicroRNAs and in-Depth Classification of …
C Yao, Q Wang, X Lu, X Chen, Z Li - Nano Letters, 2024 - ACS Publications
The primary challenge in microarray-based biological analysis lies in achieving the sensitive
and specific detection of single-molecule targets while ensuring high reproducibility. A user …
and specific detection of single-molecule targets while ensuring high reproducibility. A user …
An approach for integrating droplet generation and detection in digital polymerase chain reaction applications based on a bifunctional microfluidic cross-structure
J Wang, X Lyu, X Zhang, S Wang, W Zeng, T Yang… - Talanta, 2024 - Elsevier
Digital polymerase chain reaction (dPCR) is an approach for absolute nucleic acid
quantification with high sensitivity. Although several successful commercial dPCR devices …
quantification with high sensitivity. Although several successful commercial dPCR devices …
A ddPCR platform based on a microfluidic chip with a dual-function flow-focusing structure for sample-to-result DNA quantification analysis
X Zhang, S Wang, J Wang, X Sun, J Xue, Z Wang… - Lab on a Chip, 2024 - pubs.rsc.org
Droplet digital PCR (ddPCR) is a powerful method for absolute nucleic acid quantification
with high precision and accuracy. However, complicated operational steps have hampered …
with high precision and accuracy. However, complicated operational steps have hampered …
Evaluation of droplet digital PCR by detecting cell-free DNA in pleural effusion for the diagnosis of tuberculous pleurisy: a multicenter cohort study
F Xu, W Du, C Li, Y Li, Z Li, W Han, H Li, J Liang… - Clinical Microbiology …, 2024 - Elsevier
Objectives Tuberculous pleurisy is one of the most common extra-pulmonary tuberculosis,
but the sensitivity of conventional mycobacterial culture (Culture) or Xpert MTB/RIF assay …
but the sensitivity of conventional mycobacterial culture (Culture) or Xpert MTB/RIF assay …
Ultra-Rapid Droplet Digital PCR Enables Intraoperative Tumor Quantification
The diagnosis and treatment of tumors often depends on molecular-genetic data. However,
rapid and iterative access to molecular data is not currently feasible during surgery …
rapid and iterative access to molecular data is not currently feasible during surgery …
The Butterfly Effect of Tiny Density Difference in Microfluidic Channel
D Li, G Ni, M Wang, J Wu, H Zhang, Y Yang… - Available at SSRN … - papers.ssrn.com
Microfluidic technology plays a vital role in modern industry and advanced scientific
research due to its exceptional features. Owing to the small scales and minor density …
research due to its exceptional features. Owing to the small scales and minor density …