Second harmonic generation (SHG) microscopy is an important optical imaging technique in
a variety of applications. This article describes the history and physical principles of SHG …

Multiphoton microscopy has enabled unprecedented dynamic exploration in living
organisms. A significant challenge in biological research is the dynamic imaging of features …

Intravital microscopy (IVM) emerged and matured as a powerful tool for elucidating
pathways in biological processes. Although label-free multiphoton IVM is attractive for its …

The preparation, staining, visualization and interpretation of histological images of tissue is
well accepted as the gold standard process for the diagnosis of disease. These methods …

We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth
achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo …

Since the work of Golgi and Cajal, light microscopy has remained a key tool for
neuroscientists to observe cellular properties. Ongoing advances have enabled new …

Second harmonic generation (SHG) and third harmonic generation (THG) microscopies
have emerged as powerful imaging modalities to examine structural properties of a wide …

We review the current state of multiphoton microscopy. In particular, the requirements and
limitations associated with high-speed multiphoton imaging are considered. A description of …

Optical second-and third-harmonic generations have attracted a lot of attention in the
biomedical imaging research field recently due to their intrinsic sectioning ability and …

Second-harmonic generation (SHG) microscopy began to emerge as a high-resolution
optical imaging modality in the late 1990s, about 10 years after two-photon excited …