Mammalian genomes express two principal gene categories through RNA polymerase II-
mediated transcription: protein-coding transcription units and non-coding RNA transcription …

Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

Summary RNA polymerase II (RNAPII) pausing in early elongation is critical for gene
regulation. Paused RNAPII can be released into productive elongation by the kinase P-TEFb …

RNA abundance is a powerful indicator of the state of individual cells. Single-cell RNA
sequencing can reveal RNA abundance with high quantitative accuracy, sensitivity and …

The mechanistic understanding of nascent RNAs in transcriptional control remains limited.
Here, by a high sensitivity method methylation-inscribed nascent transcripts sequencing …

Few transcription factors have been examined for their direct roles in physically connecting
enhancers and promoters. Here acute degradation of Yin Yang 1 (YY1) in erythroid cells …

Gene expression profiling by high-throughput sequencing reveals qualitative and
quantitative changes in RNA species at steady state but obscures the intracellular dynamics …

DNA can determine where and when genes are expressed, but the full set of sequence
determinants that control gene expression is unknown. Here, we measured the …

Gene expression is controlled in a dynamic and regulated manner to allow for the consistent
and steady expression of some proteins as well as the rapidly changing production of other …

Single-cell RNA genomics technologies are revolutionizing biomedical science by profiling
single cells with unprecedented resolution, providing fundamental insights into the role of …