Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

H Zhang, L Cao, J Brodsky, I Gablech, F Xu, Z Li… - TrAC Trends in …, 2024 - Elsevier
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …

Quantification of Legionella pneumophila in building potable water systems: A meta-analysis comparing qPCR and culture-based detection methods

É Sylvestre, WJ Rhoads, TR Julian, F Hammes - PLOS Water, 2025 - journals.plos.org
Quantitative polymerase chain reaction (qPCR) offers a rapid, automated, and potentially on-
site method for quantifying L. pneumophila in building potable water systems …

SARS-CoV-2 viral titer measurements in Ontario, Canada wastewaters throughout the COVID-19 pandemic

PM D'Aoust, N Hegazy, NT Ramsay, MI Yang… - Scientific Data, 2024 - nature.com
During the COVID-19 pandemic, the Province of Ontario, Canada, launched a wastewater
surveillance program to monitor SARS-CoV-2, inspired by the early work and successful …

Pepper mild mottle virus intended for use as a process indicator for drinking water treatment: Present forms and quantitative relations to norovirus and rotavirus in …

T Miura, S Kadoya, Y Miura, H Takino, M Akiba, D Sano… - Water Research, 2024 - Elsevier
Pepper mild mottle virus (PMMoV) has been proposed as a potential indicator of human
enteric viruses in environmental water and for viral removal during drinking water treatment …

Prolonged Linear Amplification of qPCR for the Correction of Amplification Variation and the Absolute Quantification without Standard Curves

X Jiang, BB **e, K Li, F Zhou - Analytical Chemistry, 2023 - ACS Publications
The variable amplification efficiency of each thermal cycle of qPCR obeys the Poisson
distribution, and the qPCR system dynamically changes, so there must be a detection error …

Impedance-based method for the quantification of infectious SARS-CoV-2

A Fraisse, L Guillier, C Cordevant, S Le Poder… - Journal of Virological …, 2025 - Elsevier
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent
involved in the coronavirus disease 2019 (COVID-19) pandemic. The development of …

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

T Zhu, X Liu, X **ao - Applied Sciences, 2024 - mdpi.com
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been
widely used in molecular biology applications, where the reliability of the qPCR performance …

Development of novel species-specific and genus-specific primers for the detection of Babaco Mosaic Virus (BabMV)

M Albuja-Quintana, CE Armijos… - Letters in Applied …, 2024 - academic.oup.com
Babaco is a hybrid cultivar native to the Andean region of Ecuador and Colombia,
commercially attractive for its fruit. Babaco production in Ecuador faces losses from plant …

A simple method for testing and controlling inhibition in soil and sediment samples for qPCR

T Bott, G Shaw, S Gregory - Journal of Microbiological Methods, 2023 - Elsevier
The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental
samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) …

The design and application of a qPCR assay for the study of light regime as a driver of rhodopsin expression in high Arctic phytoplankton.

EK Nicholls - 2023 - duo.uio.no
Several months of darkness during polar night impose unique challenges on polar
phototrophic organisms, whereby solar energy capture via plastid-based photosystems …