The use of nanotechnology has the potential to revolutionize the detection and treatment of
cancer. Developments in protein engineering and materials science have led to the …

With the recent development of CRISPR technology, it is becoming increasingly easy to
engineer the genome. Genome-editing systems based on CRISPR, as well as transcription …

Genome-wide screening using CRISPR coupled with nuclease Cas9 (CRISPR–Cas9) is a
powerful technology for the systematic evaluation of gene function. Statistically principled …

It is extremely rare for a single experiment to be so impactful and timely that it shapes and
forecasts the experiments of the next decade. Here, we review how two such experiments …

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …

Genome-wide association studies (GWAS) have identified numerous genetic variants
associated with complex diseases, but mechanistic insights are impeded by a lack of …

The human brain has undergone rapid expansion since humans diverged from other great
apes, but the mechanism of this human-specific enlargement is still unknown. Here, we use …

Nucleases that cleave unique genomic sequences in living cells can be used for targeted
gene editing and mutagenesis. Here we develop a strategy for generating such reagents …

Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis
thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and …

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …