Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Live-cell imaging powered by computation

H Shroff, I Testa, F Jug, S Manley - Nature Reviews Molecular Cell …, 2024 - nature.com
The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

Visualizing and discovering cellular structures with super-resolution microscopy

YM Sigal, R Zhou, X Zhuang - Science, 2018 - science.org
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

Fluorescence nanoscopy in cell biology

SJ Sahl, SW Hell, S Jakobs - Nature reviews Molecular cell biology, 2017 - nature.com
Fluorescence nanoscopy uniquely combines minimally invasive optical access to the
internal nanoscale structure and dynamics of cells and tissues with molecular detection …

Amplified stimulated emission in upconversion nanoparticles for super-resolution nanoscopy

Y Liu, Y Lu, X Yang, X Zheng, S Wen, F Wang, X Vidal… - Nature, 2017 - nature.com
Lanthanide-doped glasses and crystals are attractive for laser applications because the
metastable energy levels of the trivalent lanthanide ions facilitate the establishment of …

Nanoparticles for super-resolution microscopy and single-molecule tracking

D **, P **, B Wang, L Zhang, J Enderlein… - Nature …, 2018 - nature.com
We review the use of luminescent nanoparticles in super-resolution imaging and single-
molecule tracking, and showcase novel approaches to super-resolution imaging that …

Stimulated emission depletion microscopy

H Blom, J Widengren - Chemical reviews, 2017 - ACS Publications
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles

Q Zhan, H Liu, B Wang, Q Wu, R Pu, C Zhou… - Nature …, 2017 - nature.com
Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging
modality for life science studies. Conventionally, stimulated emission depletion requires a …

The 2015 super-resolution microscopy roadmap

SW Hell, SJ Sahl, M Bates, X Zhuang… - Journal of Physics D …, 2015 - iopscience.iop.org
Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics

D Li, L Shao, BC Chen, X Zhang, M Zhang, B Moses… - Science, 2015 - science.org
INTRODUCTION Various methods of super-resolution (SR) fluorescence microscopy have
the potential to follow the dynamic nanoscale interactions of specific macromolecular …