Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …

Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of
cell types and organisms. The newly-developed transcription activator-like effector …

Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis
thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and …

RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune
system known as CRISPR/Cas, enable targeted genome engineering in cells and …

Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …

Gene therapy has historically been defined as the addition of new genes to human cells.
However, the recent advent of genome-editing technologies has enabled a new paradigm in …

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …

Transcription activator-like effector nucleases (TALENs) are programmable nucleases that
join FokI endonuclease with the modular DNA-binding domain of TALEs. Although zinc …

Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …

We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of
genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable …