[PDF][PDF] Going around the Kok cycle of the water oxidation reaction with femtosecond X-ray crystallography

A Bhowmick, PS Simon, I Bogacz, R Hussein, M Zhang… - IUCrJ, 2023 - journals.iucr.org
The water oxidation reaction in photosystem II (PS II) produces most of the molecular oxygen
in the atmosphere, which sustains life on Earth, and in this process releases four electrons …

[HTML][HTML] All polymer microfluidic chips—A fixed target sample delivery workhorse for serial crystallography

KK Gu, Z Liu, SR Narayanasamy, ML Shelby, N Chan… - …, 2023 - pubs.aip.org
The development of x-ray free electron laser (XFEL) light sources and serial crystallography
methodologies has led to a revolution in protein crystallography, enabling the determination …

An ultraviolet-driven rescue pathway for oxidative stress to eye lens protein human gamma-D crystallin

JA Hill, Y Nyathi, S Horrell, D von Stetten… - Communications …, 2024 - nature.com
Human gamma-D crystallin (HGD) is a major constituent of the eye lens. Aggregation of
HGD contributes to cataract formation, the leading cause of blindness worldwide. It is unique …

[HTML][HTML] Sheet-on-sheet fixed target data collection devices for serial crystallography at synchrotron and XFEL sources

RB Doak, RL Shoeman, A Gorel, S Nizinski… - Applied …, 2024 - journals.iucr.org
Serial crystallography (SX) efficiently distributes over many crystals the radiation dose
absorbed during diffraction data acquisition, enabling structure determination of samples at …

Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron

J Orlans, SL Rose, G Ferguson, M Oscarsson… - Communications …, 2025 - nature.com
Serial macromolecular crystallography has become a powerful method to reveal room
temperature structures of biological macromolecules and perform time-resolved studies …

A redox switch allows binding of Fe(II) and Fe(III) ions in the cyanobacterial iron-binding protein FutA from Prochlorococcus

R Bolton, MM Machelett, J Stubbs… - Proceedings of the …, 2024 - National Acad Sciences
The marine cyanobacterium Prochlorococcus is a main contributor to global photosynthesis,
whilst being limited by iron availability. Cyanobacterial genomes generally encode two …

Small-rotative fixed-target serial synchrotron crystallography (SR-FT-SSX) for molecular crystals

SG Lewis, BA Coulson, AJ Warren… - Communications …, 2024 - nature.com
The increasing availability of ultrabright Light Sources is facilitating the study of smaller
crystals at faster timescales but with an increased risk of severe X-ray damage, leading to …

Double crossed? Structural and computational studies of an unusual crosslinked heme in Methylococcus capsulatus cytochrome P460

HE Pfalzgraf, AG Rao, K Sen, HR Adams, M Edwards… - bioRxiv, 2024 - biorxiv.org
Cytochromes P460 oxidise hydroxylamine within the nitrogen cycle and contain as their
active site an unusual catalytic c-type heme where the porphyrin is cross-linked to the …

[PDF][PDF] Serial macromolecular crystallography at ALBA Synchrotron Light Source

JM Martin-Garcia, S Botha, H Hu… - Journal of …, 2022 - journals.iucr.org
The increase in successful adaptations of serial crystallography at synchrotron radiation
sources continues. To date, the number of serial synchrotron crystallography (SSX) …

Use of fixed targets for serial crystallography

S Jaho, D Axford, DH Gu, MA Hough… - Methods in …, 2024 - pubmed.ncbi.nlm.nih.gov
In serial crystallography, large numbers of microcrystals are sequentially delivered to an X-
ray beam and a diffraction pattern is obtained from each crystal. This serial approach was …