Metagenomics for pathogen detection in public health
Traditional pathogen detection methods in public health infectious disease surveillance rely
upon the identification of agents that are already known to be associated with a particular …
upon the identification of agents that are already known to be associated with a particular …
Microbial growth under limiting conditions-future perspectives
JM Gonzalez, B Aranda - Microorganisms, 2023 - mdpi.com
Microorganisms rule the functioning of our planet and each one of the individual
macroscopic living creature. Nevertheless, microbial activity and growth status have always …
macroscopic living creature. Nevertheless, microbial activity and growth status have always …
Consistent and correctable bias in metagenomic sequencing experiments
Marker-gene and metagenomic sequencing have profoundly expanded our ability to
measure biological communities. But the measurements they provide differ from the truth …
measure biological communities. But the measurements they provide differ from the truth …
How quantitative is metabarcoding: A meta‐analytical approach
Metabarcoding has been used in a range of ecological applications such as taxonomic
assignment, dietary analysis and the analysis of environmental DNA. However, after a …
assignment, dietary analysis and the analysis of environmental DNA. However, after a …
Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling
The use of relative abundance data from next generation sequencing (NGS) can lead to
misinterpretations of microbial community structures, as the increase of one taxon leads to …
misinterpretations of microbial community structures, as the increase of one taxon leads to …
Metagenomic 16S rDNA I llumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities
R Logares, S Sunagawa, G Salazar… - Environmental …, 2014 - Wiley Online Library
Sequencing of 16S rDNA polymerase chain reaction (PCR) amplicons is the most common
approach for investigating environmental prokaryotic diversity, despite the known biases …
approach for investigating environmental prokaryotic diversity, despite the known biases …
Accurate multiplexing and filtering for high-throughput amplicon-sequencing
Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …
[PDF][PDF] Towards quantitative metabarcoding of eukaryotic plankton: an approach to improve 18S rRNA gene copy number bias
Plankton metabarcoding is increasingly implemented in marine ecosystem assessments
and is more cost-efficient and less time-consuming than monitoring based on microscopy …
and is more cost-efficient and less time-consuming than monitoring based on microscopy …
Large-scale biomonitoring of remote and threatened ecosystems via high-throughput sequencing
Biodiversity metrics are critical for assessment and monitoring of ecosystems threatened by
anthropogenic stressors. Existing sorting and identification methods are too expensive and …
anthropogenic stressors. Existing sorting and identification methods are too expensive and …
Dandruff is associated with the conjoined interactions between host and microorganisms
Z Xu, Z Wang, C Yuan, X Liu, F Yang, T Wang… - Scientific reports, 2016 - nature.com
Dandruff is an unpleasant scalp disorder common to human populations. In this study, we
systematically investigated the intra-and inter-associations among dandruff, physiological …
systematically investigated the intra-and inter-associations among dandruff, physiological …