Metagenomics for pathogen detection in public health

RR Miller, V Montoya, JL Gardy, DM Patrick, P Tang - Genome medicine, 2013 - Springer
Traditional pathogen detection methods in public health infectious disease surveillance rely
upon the identification of agents that are already known to be associated with a particular …

Microbial growth under limiting conditions-future perspectives

JM Gonzalez, B Aranda - Microorganisms, 2023 - mdpi.com
Microorganisms rule the functioning of our planet and each one of the individual
macroscopic living creature. Nevertheless, microbial activity and growth status have always …

Consistent and correctable bias in metagenomic sequencing experiments

MR McLaren, AD Willis, BJ Callahan - Elife, 2019 - elifesciences.org
Marker-gene and metagenomic sequencing have profoundly expanded our ability to
measure biological communities. But the measurements they provide differ from the truth …

How quantitative is metabarcoding: A meta‐analytical approach

PD Lamb, E Hunter, JK Pinnegar, S Creer… - Molecular …, 2019 - Wiley Online Library
Metabarcoding has been used in a range of ecological applications such as taxonomic
assignment, dietary analysis and the analysis of environmental DNA. However, after a …

Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling

C Jian, P Luukkonen, H Yki-Järvinen, A Salonen… - PloS one, 2020 - journals.plos.org
The use of relative abundance data from next generation sequencing (NGS) can lead to
misinterpretations of microbial community structures, as the increase of one taxon leads to …

Metagenomic 16S rDNA I llumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities

R Logares, S Sunagawa, G Salazar… - Environmental …, 2014 - Wiley Online Library
Sequencing of 16S rDNA polymerase chain reaction (PCR) amplicons is the most common
approach for investigating environmental prokaryotic diversity, despite the known biases …

Accurate multiplexing and filtering for high-throughput amplicon-sequencing

P Esling, F Lejzerowicz, J Pawlowski - Nucleic acids research, 2015 - academic.oup.com
Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …

[PDF][PDF]  Towards quantitative metabarcoding of eukaryotic plankton: an approach to improve 18S rRNA gene copy number bias

JL Martin, I Santi, P Pitta, U John… - Metabarcoding and …, 2022 - mbmg.pensoft.net
Plankton metabarcoding is increasingly implemented in marine ecosystem assessments
and is more cost-efficient and less time-consuming than monitoring based on microscopy …

Large-scale biomonitoring of remote and threatened ecosystems via high-throughput sequencing

JF Gibson, S Shokralla, C Curry, DJ Baird, WA Monk… - PloS one, 2015 - journals.plos.org
Biodiversity metrics are critical for assessment and monitoring of ecosystems threatened by
anthropogenic stressors. Existing sorting and identification methods are too expensive and …

Dandruff is associated with the conjoined interactions between host and microorganisms

Z Xu, Z Wang, C Yuan, X Liu, F Yang, T Wang… - Scientific reports, 2016 - nature.com
Dandruff is an unpleasant scalp disorder common to human populations. In this study, we
systematically investigated the intra-and inter-associations among dandruff, physiological …