An efficient genome-scale editing tool is required for construction of industrially useful
microbes. We describe a targeted, continual multigene editing strategy that was applied to …

Populations of surface‐attached microorganisms comprising either single or multiple
species are commonly referred to as biofilms. Using a simple assay for the initiation of …

With the widespread appearance of antibiotic-resistant bacteria, there is an increasing
demand for novel strategies to control infectious diseases. Furthermore, it has become …

We report the identification of an ATP‐binding cassette (ABC) transporter and an associated
large cell‐surface protein that are required for biofilm formation by Pseudomonas …

The chromosomally encoded arsenical resistance (ars) operon subcloned into a multicopy
plasmid was found to confer a moderate level of resistance to arsenite and antimonite in …

Publisher Summary The widely used phage isolation method, described in Adams, includes
a chloroform treatment step. As a result, bacterial viruses have been considered to be …

Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably
maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The …

A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with
pBR322 and its derivatives (especially the pUC family of vectors), is described. They all …

Escherichia coli ST131 is a globally disseminated, multidrug resistant clone responsible for
a high proportion of urinary tract and bloodstream infections. The rapid emergence and …

An improved method for allele replacement in Pseudomonas aeruginosa was developed.
The two main ingredients of the method are:(i) novel ColE1‐type cloning vectors derived …