Polo-like kinase 1 (PLK1) plays an important role in a variety of cellular functions, including
the regulation of mitosis, DNA replication, autophagy, and the epithelial–mesenchymal …

The polo-like kinase 1 (PLK1) acts in concert with cyclin-dependent kinase 1–cyclin B1 and
Aurora kinases to orchestrate a wide range of critical cell cycle events. Because PLK1 has …

Antibodies are key reagents to investigate cellular processes. The development of
recombinant antibodies and binders derived from natural protein scaffolds has expanded …

Antibodies have been the paradigm of binding proteins with desired specificities for more
than one century and during the past decade their recombinant or humanized versions have …

Antibodies have long been regarded as the only class of binding proteins. With the
emergence of protein engineering techniques, new binding proteins based on alternative …

The preparation of diffraction quality crystals remains the major bottleneck in
macromolecular X-ray crystallography. A crystallization chaperone is an auxiliary protein …

Designed ankyrin repeat proteins (DARPins) have been developed into a robust and
versatile scaffold for binding proteins. High-affinity binders are routinely selected by …

Binding proteins are typically isolated from combinatorial libraries of scaffold proteins using
one of the many library screening tools available, such as phage display, yeast surface …

Abstract Polo-like kinase 1 (PLK1) is a conserved mitotic serine-threonine protein kinase,
functions as a regulatory protein, and is involved in the progression of the mitotic cycle. It …

The use of combinatorial protein engineering to design proteins with novel binding
specificities and desired properties has evolved into a powerful technology, resulting in the …