The remarkable metabolic diversity observed in nature has provided a foundation for
sustainable production of a wide array of valuable molecules. However, transferring the …

In the past decades, the broad selection of CRISPR-Cas systems has revolutionized
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …

Cell-free protein synthesis systems that can be lyophilized for long-term, non-refrigerated
storage and transportation have the potential to enable decentralized biomanufacturing …

Carbon-negative synthesis of biochemical products has the potential to mitigate global CO2
emissions. An attractive route to do this is the reverse β-oxidation (r-BOX) pathway coupled …

Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Metabolism has long been considered as a relatively stiff set of biochemical reactions. This
somewhat outdated and dogmatic view has been challenged over the last years, as multiple …

Climate change poses a significant threat to global agriculture, necessitating innovative
solutions. Plant synthetic biology, particularly chloroplast engineering, holds promise as a …

Nature's two redox cofactors, nicotinamide adenine dinucleotide (NAD+) and nicotinamide
adenine dinucleotide phosphate (NADP+), are held at different reduction potentials, driving …

Engineering regulatory parts for improved performance in genetic programs has played a
pivotal role in the development of the synthetic biology cell programming toolbox. Here, we …

Building and optimizing biosynthetic pathways in engineered cells holds promise to address
societal needs in energy, materials, and medicine, but it is often time-consuming. Cell-free …