Decoding biology with massively parallel reporter assays and machine learning

A La Fleur, Y Shi, G Seelig - Genes & Development, 2024 - genesdev.cshlp.org
Massively parallel reporter assays (MPRAs) are powerful tools for quantifying the impacts of
sequence variation on gene expression. Reading out molecular phenotypes with …

How our brains are built: emerging approaches to understand human-specific features

ND Moss, D Lollis, DL Silver - Current Opinion in Genetics & Development, 2024 - Elsevier
Understanding what makes us uniquely human is a long-standing question permeating
fields from genomics, neuroscience, and developmental biology to medicine. The discovery …

Multiplex generation and single-cell analysis of structural variants in mammalian genomes

S **lay, JB Lalanne, RM Daza, S Kottapalli… - Science, 2025 - science.org
Studying the functional consequences of structural variants (SVs) in mammalian genomes is
challenging because (i) SVs arise much less commonly than single-nucleotide variants or …

[HTML][HTML] Just a SNP away: The future of in vivo massively parallel reporter assay

KN Degner, JL Bell, SD Jones, H Won - Cell Insight, 2024 - Elsevier
The human genome is largely noncoding, yet the field is still gras** to understand how
noncoding variants impact transcription and contribute to disease etiology. The massively …

Targeted mutagenesis of specific genomic DNA sequences in animals for the in vivo generation of variant libraries

J Falo-Sanjuan, Y Diaz-Tirado, MA Turner, J Davis… - …, 2024 - pmc.ncbi.nlm.nih.gov
Understanding how the number, placement and affinity of transcription factor binding sites
dictates gene regulatory programs remains a major unsolved challenge in biology …

[HTML][HTML] Deciphering the functional impact of Alzheimer's Disease-associated variants in resting and proinflammatory immune cells

ML Bond, IY Quiroga-Barber, S D'Costa, Y Wu… - medRxiv, 2024 - pmc.ncbi.nlm.nih.gov
Genome-wide association studies have identified loci associated with Alzheimer's Disease
(AD), but identifying the exact causal variants and genes at each locus is challenging due to …

Spatial-scERA: A method for reconstructing spatial single-cell enhancer activity in multicellular organisms

B Alberti, S Vincent, I Stévant, D Lajoignie, H Tarayre… - bioRxiv, 2024 - biorxiv.org
Enhancers play an essential role in developmental processes by orchestrating the spatial
and temporal regulation of gene expression. However, map** the location of these …