Targeted proteomics via selected reaction monitoring (SRM) or parallel reaction monitoring
(PRM) enables fast and sensitive detection of a preselected set of target peptides. However …

The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …

Achieving sufficient coverage of regulatory phosphorylation sites by mass spectrometry (MS)-
based phosphoproteomics for signaling pathway reconstitution is challenging, especially …

Owing to its roles in cellular signal transduction, protein phosphorylation plays critical roles
in myriad cell processes. That said, detecting and quantifying protein phosphorylation has …

Introduction Many pathologies, including cancer, have been associated with aberrant
phosphorylation-mediated signaling networks that drive altered cell proliferation, migration …

Mass spectrometry-based phosphoproteomics is currently the leading methodology for the
study of global kinase signaling. The scientific community is continuously releasing …

Sample preparation for mass spectrometry‐based proteomics has many tedious and time‐
consuming steps that can introduce analytical errors. In particular, the steps around the …

Introduction Protein phosphorylation is a critical post-translational modification involved in
the regulation of numerous cellular processes from signal transduction to modulation of …

Proteins are the molecular effectors of the information encoded in the genome. Proteomics
aims at understanding the molecular functions of proteins in their biological context. In …

Absolute quantification measurements (copies per cell) of peptide major histocompatibility
complex (pMHC) antigens are necessary to inform targeted immunotherapy drug design; …