The properties, biotechnologies, and applications of antifreeze proteins

H **ang, X Yang, L Ke, Y Hu - International journal of biological …, 2020‏ - Elsevier
By natural selection, organisms evolve different solutions to cope with extremely cold
weather. The emergence of an antifreeze protein gene is one of the most momentous …

Antifreeze proteins and their biomimetics for cell cryopreservation: Mechanism, function and application-A review

X Wu, F Yao, H Zhang, J Li - International Journal of Biological …, 2021‏ - Elsevier
Cell-based therapy is a promising technology for intractable diseases and health care
applications, in which cryopreservation has become an essential procedure to realize the …

Crystal structure of an insect antifreeze protein reveals ordered waters on the ice-binding surface

Q Ye, R Eves, RL Campbell, PL Davies - Biochemical Journal, 2020‏ - portlandpress.com
Antifreeze proteins (AFPs) are characterized by their ability to adsorb to the surface of ice
crystals and prevent any further crystal growth. AFPs have independently evolved for this …

Inhibition of bacterial ice nucleators is not an intrinsic property of antifreeze proteins

R Schwidetzky, AT Kunert, M Bonn… - The Journal of …, 2020‏ - ACS Publications
Cold-adapted organisms use antifreeze proteins (AFPs) or ice-nucleating proteins (INPs) for
the survival in freezing habitats. AFPs have been reported to be able to inhibit the activity of …

Genetic algorithm approach for the optimization of protein antifreeze activity using molecular simulations

DJ Kozuch, FH Stillinger… - Journal of Chemical …, 2020‏ - ACS Publications
Antifreeze proteins (AFPs) are of much interest for their ability to inhibit ice growth at low
concentrations. In this work, we present a genetic algorithm for the in silico design of AFP …

Polyproline type II helical antifreeze proteins are widespread in Collembola and likely originated over 400 million years ago in the Ordovician Period

CL Scholl, M Holmstrup, LA Graham, PL Davies - Scientific Reports, 2023‏ - nature.com
Antifreeze proteins (AFPs) bind to ice crystals to prevent organisms from freezing. A diversity
of AFP folds has been found in fish and insects, including alpha helices, globular proteins …

Protein engineering of antifreeze proteins reveals that their activity scales with the area of the ice‐binding site

CL Scholl, PL Davies - FEBS letters, 2023‏ - Wiley Online Library
Antifreeze proteins (AFPs) protect organisms from freezing by binding to ice crystals to
prevent their growth. Here, we have investigated how the area of an AFP's ice‐binding site …

Antifreeze protein complements cryoprotective dehydration in the freeze-avoiding springtail Megaphorura arctica

LA Graham, ME Boddington, M Holmstrup… - Scientific Reports, 2020‏ - nature.com
The springtail, Megaphorura arctica, is freeze-avoiding and survives sub-zero temperatures
by cryoprotective dehydration. At the onset of dehydration there is some supercooling of …

Interfacial Ice Density Fluctuations Inform Surface Ice-Philicity

Z Vicars, J Choi, SM Marks, RC Remsing… - The Journal of …, 2024‏ - ACS Publications
The propensity of a surface to nucleate ice or bind to ice is governed by its ice-philicity─ its
relative preference for ice over liquid water. However, the relationship between the features …

New Cysteine-Rich Ice-Binding Protein Secreted from Antarctic Microalga, Chloromonas sp.

W Jung, RL Campbell, Y Gwak, JI Kim, PL Davies… - PLoS …, 2016‏ - journals.plos.org
Many microorganisms in Antarctica survive in the cold environment there by producing ice-
binding proteins (IBPs) to control the growth of ice around them. An IBP from the Antarctic …