Cross-linking mass spectrometry for investigating protein conformations and protein–protein interactions─ a method for all seasons
Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …
Cross-linking mass spectrometry: methods and applications in structural, molecular and systems biology
Over the past decade, cross-linking mass spectrometry (CLMS) has developed into a robust
and flexible tool that provides medium-resolution structural information. CLMS data provide …
and flexible tool that provides medium-resolution structural information. CLMS data provide …
Protein structure prediction with in-cell photo-crosslinking mass spectrometry and deep learning
While AlphaFold2 can predict accurate protein structures from the primary sequence,
challenges remain for proteins that undergo conformational changes or for which few …
challenges remain for proteins that undergo conformational changes or for which few …
In-cell architecture of an actively transcribing-translating expressome
Structural biology studies performed inside cells can capture molecular machines in action
within their native context. In this work, we developed an integrative in-cell structural …
within their native context. In this work, we developed an integrative in-cell structural …
[HTML][HTML] Structure and mechanism of the RNA polymerase II transcription machinery
AC Schier, DJ Taatjes - Genes & development, 2020 - genesdev.cshlp.org
Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …
Cross-linking mass spectrometry (XL-MS): An emerging technology for interactomics and structural biology
Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …
and the assembly of multisubunit protein complexes that represent the functional …
Structural basis of transcription initiation by RNA polymerase II
Transcription of eukaryotic protein-coding genes commences with the assembly of a
conserved initiation complex, which consists of RNA polymerase II (Pol II) and the general …
conserved initiation complex, which consists of RNA polymerase II (Pol II) and the general …
Principles for integrative structural biology studies
Integrative structure determination is a powerful approach to modeling the structures of
biological systems based on data produced by multiple experimental and theoretical …
biological systems based on data produced by multiple experimental and theoretical …
Crosslinking and mass spectrometry: an integrated technology to understand the structure and function of molecular machines
A Leitner, M Faini, F Stengel, R Aebersold - Trends in biochemical …, 2016 - cell.com
In recent years, chemical crosslinking of protein complexes and the identification of
crosslinked residues by mass spectrometry (XL-MS; sometimes abbreviated as CX-MS) has …
crosslinked residues by mass spectrometry (XL-MS; sometimes abbreviated as CX-MS) has …
Cryo-EM: a unique tool for the visualization of macromolecular complexity
3D cryo-electron microscopy (cryo-EM) is an expanding structural biology technique that has
recently undergone a quantum leap progression in its achievable resolution and its …
recently undergone a quantum leap progression in its achievable resolution and its …