Single-cell omics: experimental workflow, data analyses and applications

F Sun, H Li, D Sun, S Fu, L Gu, X Shao, Q Wang… - Science China Life …, 2024 - Springer
Cells are the fundamental units of biological systems and exhibit unique development
trajectories and molecular features. Our exploration of how the genomes orchestrate the …

WebCSEA: web-based cell-type-specific enrichment analysis of genes

Y Dai, R Hu, A Liu, KS Cho, AM Manuel… - Nucleic acids …, 2022 - academic.oup.com
Human complex traits and common diseases show tissue-and cell-type-specificity. Recently,
single-cell RNA sequencing (scRNA-seq) technology has successfully depicted cellular …

Recruitment of CXCR4+ type 1 innate lymphoid cells distinguishes sarcoidosis from other skin granulomatous diseases

S Sati, J Huang, AE Kersh, P Jones, O Ahart… - The Journal of Clinical …, 2024 - jci.org
Sarcoidosis is a multiorgan granulomatous disease that lacks diagnostic biomarkers and
targeted treatments. Using blood and skin from patients with sarcoid and non-sarcoid skin …

Single-cell multiomics decodes regulatory programs for mouse secondary palate development

F Yan, A Suzuki, C Iwaya, G Pei, X Chen… - Nature …, 2024 - nature.com
Perturbations in gene regulation during palatogenesis can lead to cleft palate, which is
among the most common congenital birth defects. Here, we perform single-cell multiome …

scGWAS: landscape of trait-cell type associations by integrating single-cell transcriptomics-wide and genome-wide association studies

P Jia, R Hu, F Yan, Y Dai, Z Zhao - Genome biology, 2022 - Springer
Background The rapid accumulation of single-cell RNA sequencing (scRNA-seq) data
presents unique opportunities to decode the genetically mediated cell-type specificity in …

CellSTAR: a comprehensive resource for single-cell transcriptomic annotation

Y Zhang, H Sun, W Zhang, T Fu, S Huang… - Nucleic Acids …, 2024 - academic.oup.com
Large-scale studies of single-cell sequencing and biological experiments have successfully
revealed expression patterns that distinguish different cell types in tissues, emphasizing the …

Automatic cell type annotation using marker genes for single-cell RNA sequencing data

Y Chen, S Zhang - Biomolecules, 2022 - mdpi.com
Recent advancement in single-cell RNA sequencing (scRNA-seq) technology is gaining
more and more attention. Cell type annotation plays an essential role in scRNA-seq data …

Identifying candidate genes and drug targets for Alzheimer's disease by an integrative network approach using genetic and brain region-specific proteomic data

A Liu, AM Manuel, Y Dai, BS Fernandes… - Human Molecular …, 2022 - academic.oup.com
Genome-wide association studies (GWAS) have identified more than 75 genetic variants
associated with Alzheimer's disease (ad). However, how these variants function and impact …

Integrated single‐cell and spatial transcriptomic profiling reveals higher intratumour heterogeneity and epithelial–fibroblast interactions in recurrent bladder cancer

ZD Shi, Z Sun, ZB Zhu, X Liu, JZ Chen… - Clinical and …, 2023 - Wiley Online Library
Background Recurrent bladder cancer is the most common type of urinary tract malignancy;
nevertheless, the mechanistic basis for its recurrence is uncertain. Innovative technologies …

Methods for cell-type annotation on scRNA-seq data: A recent overview

K Lazaros, P Vlamos, AG Vrahatis - Journal of Bioinformatics and …, 2023 - World Scientific
The evolution of single-cell technology is ongoing, continually generating massive amounts
of data that reveal many mysteries surrounding intricate diseases. However, their drawbacks …