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Engineering a robust Cas12i3 variant‐mediated wheat genome editing system
W Wang, L Yan, J Li, C Zhang, Y He… - Plant Biotechnology …, 2024 - Wiley Online Library
Summary Wheat (Triticum aestivum L., 2 n= 6 x= 42, AABBDD) is one of the most important
food crops in the world. CRISPR/Cas12i3, which belongs to the type V‐I Cas system, has …
food crops in the world. CRISPR/Cas12i3, which belongs to the type V‐I Cas system, has …
A quantitative assay for the efficiency of RNA‐guided genome editing in plants
M Bircheneder, T Schreiber, A Tissier… - The Plant …, 2024 - Wiley Online Library
SUMMARY RNA‐guided endonucleases originating from the bacterial CRISPR/Cas system
are a versatile tool for targeted gene editing. To determine the functional relevance of a …
are a versatile tool for targeted gene editing. To determine the functional relevance of a …
[HTML][HTML] A nucleoside signal generated by a fungal endophyte regulates host cell death and promotes root colonization
The intracellular colonization of plant roots by the beneficial fungal endophyte Serendipita
indica follows a biphasic strategy, including a host cell death phase that enables successful …
indica follows a biphasic strategy, including a host cell death phase that enables successful …
Efficient scar-free knock-ins of several kilobases in plants by engineered CRISPR-Cas endonucleases
T Schreiber, A Prange, P Schäfer, T Iwen, R Grützner… - Molecular Plant, 2024 - cell.com
In plants and mammals, non-homologous end-joining is the dominant pathway to repair
DNA double-strand breaks, making it challenging to generate knock-in events. In this study …
DNA double-strand breaks, making it challenging to generate knock-in events. In this study …
Versatile cloning strategy for efficient multigene editing in Arabidopsis
Z Li, J Huard, EM Bayer, V Wattelet-Boyer - Bio-protocol, 2024 - bio-protocol.org
Background CRISPR-Cas9 technology, which serves as a powerful genome editing tool, is
based on the bacterial RNA-guided CRISPR-Cas9 system [1]. The editing process involves …
based on the bacterial RNA-guided CRISPR-Cas9 system [1]. The editing process involves …