Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety of
methods, such as Electrospray Ionization-Mass Spectrometry (ESI-MS) or Matrix-Assisted …

A critical step in proteomics analysis is the optimal extraction and processing of protein
material to ensure the highest sensitivity in downstream detection. Achieving this requires a …

The gut microbiota is the largest and most complex microbial community in the human body.
Host-gut microbiota interactions have significant implications on health and disease. The …

In order to obtain a systems‐level understanding of a complex biological system, detailed
proteome information is essential. Despite great progress in proteomics technologies …

Mass spectrometry (MS)-based proteomics typically employs multistep sample-preparation
workflows that are subject to sample contamination and loss. We report an in-StageTip …

Mass spectrometry (MS)-based ubiquitinomics provides system-level understanding of
ubiquitin signaling. Here we present a scalable workflow for deep and precise in vivo …

Carbon fixation by chemoautotrophic microorganisms in the dark ocean has a major impact
on global carbon cycling and ecological relationships in the ocean's interior, but the relevant …

Robust, efficient, and reproducible protein extraction and sample processing is a key step for
bottom-up proteomics analyses. While many sample preparation protocols for mass …

The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a
manner that enables efficient protein digestion for subsequent mass spectrometric analysis …

Bottom-up proteomic strategies rely on efficient digestion of proteins into peptides for mass
spectrometry analysis. In-solution and filter-based strategies are commonly used for …