Directed Evolution of Protoglobin Optimizes the Enzyme Electric Field

SS Chaturvedi, S Vargas, P Ajmera… - Journal of the …, 2024 - ACS Publications
To unravel why computational design fails in creating viable enzymes, while directed
evolution (DE) succeeds, our research delves into the laboratory evolution of protoglobin …

[HTML][HTML] Modeling protease-sensitive human pancreatic lipase mutations in the mouse ortholog

G Hoffka, S Mhana, M Vas, V Toldi, JA Mótyán… - Journal of Biological …, 2024 - Elsevier
Pancreatic lipase (PNLIP) is the major lipolytic enzyme secreted by the pancreas. A recent
study identified human PNLIP variants P245A, I265R, F300L, S304F, and F314L in …

[HTML][HTML] Predicting fragment binding modes using customized Lennard-Jones potentials in short molecular dynamics simulations

C Vorreiter, D Robaa, W Sippl - Computational and Structural …, 2025 - Elsevier
Reliable in silico prediction of fragment binding modes remains a challenge in current drug
design research. Due to their small size and generally low binding affinity, fragments can …

[HTML][HTML] Hydrogenation Versus Hydrosilylation: The Substantial Impact of a Palladium Capsule on the Catalytic Outcome

M Steinmetz, R Schurhammer, C Gourlaouen… - Molecules, 2024 - mdpi.com
A palladium capsule, made of three cavitands, namely P, P-dichlorido {5, 17-bis [5-
(diphenylphosphanyl)-4 (24), 6 (10), 12 (16), 18 (22)-tetramethylenedioxy-2, 8, 14, 20 …

[HTML][HTML] Computational insights into the circular permutation roles on ConA binding and structural stability

VJS Osterne, VR Pinto-Junior, MV Oliveira… - Current Research in …, 2024 - Elsevier
The mechanisms behind Concanavalin A (ConA) circular permutation have been under
investigation since 1985. Although a vast amount of information is available about this lectin …

[HTML][HTML] Conformational analysis of the IQSEC2 protein by statistical thermodynamics

M Shokhen, A Albeck, V Borisov, Y Israel… - Current Research in …, 2024 - Elsevier
Mutations in the IQSEC2 gene result in severe intellectual disability, epilepsy and autism.
The primary function of IQSEC2 is to serve as a guanine exchange factor (GEF) controlling …

Comparison of Machine-Learning and Classical Force Fields in Simulating the Solvation of Small Organic Molecules in Acetonitrile

S Xun, F Liu - 2023 - chemrxiv.org
Machine learning force fields (MLFFs) have emerged as a new method for molecular
simulation that combines the accuracy of ab initio approaches with the computational …

Redox-coupled protonation of respiratory complex I: The hydrophilic domain

V Couch, D Popovic, A Stuchebrukhov - Biophysical journal, 2011 - cell.com
Respiratory complex I, NADH: ubiquinone oxidoreductase, is a large and complex integral
membrane enzyme found in respiring bacteria and mitochondria. It is responsible in part for …