CRISPR/Cas genome editing and precision plant breeding in agriculture

K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants

SSA Zaidi, A Mahas, H Vanderschuren, MM Mahfouz - Genome biology, 2020 - Springer
To meet increasing global food demand, breeders and scientists aim to improve the yield
and quality of major food crops. Plant diseases threaten food security and are expected to …

Designing future crops: challenges and strategies for sustainable agriculture

Z Tian, JW Wang, J Li, B Han - The Plant Journal, 2021 - Wiley Online Library
Crop production is facing unprecedented challenges. Despite the fact that the food supply
has significantly increased over the past half‐century,~ 8.9 and 14.3% people are still …

Heat and drought stresses in crops and approaches for their mitigation

M Lamaoui, M Jemo, R Datla, F Bekkaoui - Frontiers in chemistry, 2018 - frontiersin.org
Drought and heat are major abiotic stresses that reduce crop productivity and weaken global
food security, especially given the current and growing impacts of climate change and …

The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

Applications and potential of genome editing in crop improvement

Y Zhang, K Massel, ID Godwin, C Gao - Genome biology, 2018 - Springer
Genome-editing tools provide advanced biotechnological techniques that enable the
precise and efficient targeted modification of an organism's genome. Genome-editing …

CRISPR–Act3. 0 for highly efficient multiplexed gene activation in plants

C Pan, X Wu, K Markel, AA Malzahn, N Kundagrami… - Nature Plants, 2021 - nature.com
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …

A multipurpose toolkit to enable advanced genome engineering in plants

T Čermák, SJ Curtin, J Gil-Humanes, R Čegan… - The Plant …, 2017 - academic.oup.com
We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …

CRISPR/Cas9 in genome editing and beyond

H Wang, M La Russa, LS Qi - Annual review of biochemistry, 2016 - annualreviews.org
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …

Progress and prospects in plant genome editing

K Yin, C Gao, JL Qiu - Nature plants, 2017 - nature.com
The emergence of sequence-specific nucleases that enable genome editing is
revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome …