The BRCT domain of PARP1 binds intact DNA and mediates intrastrand transfer
PARP1 is a key player in the response to DNA damage and is the target of clinical inhibitors
for the treatment of cancers. Binding of PARP1 to damaged DNA leads to activation wherein …
for the treatment of cancers. Binding of PARP1 to damaged DNA leads to activation wherein …
Characterization of proteins by size-exclusion chromatography coupled to multi-angle light scattering (SEC-MALS)
Analytical size-exclusion chromatography (SEC), commonly used for the determination of
the molecular weight of proteins and protein-protein complexes in solution, is a relative …
the molecular weight of proteins and protein-protein complexes in solution, is a relative …
Structure of histone-based chromatin in Archaea
Small basic proteins present in most Archaea share a common ancestor with the eukaryotic
core histones. We report the crystal structure of an archaeal histone-DNA complex. DNA …
core histones. We report the crystal structure of an archaeal histone-DNA complex. DNA …
CAF-1 deposits newly synthesized histones during DNA replication using distinct mechanisms on the leading and lagging strands
C Rouillon, BV Eckhardt, L Kollenstart… - Nucleic acids …, 2023 - academic.oup.com
During every cell cycle, both the genome and the associated chromatin must be accurately
replicated. Chromatin Assembly Factor-1 (CAF-1) is a key regulator of chromatin replication …
replicated. Chromatin Assembly Factor-1 (CAF-1) is a key regulator of chromatin replication …
CENP-N promotes the compaction of centromeric chromatin
The histone variant CENP-A is the epigenetic determinant for the centromere, where it is
interspersed with canonical H3 to form a specialized chromatin structure that nucleates the …
interspersed with canonical H3 to form a specialized chromatin structure that nucleates the …
Histone Parylation factor 1 contributes to the inhibition of PARP1 by cancer drugs
Abstract Poly-(ADP-ribose) polymerase 1 and 2 (PARP1 and PARP2) are key enzymes in
the DNA damage response. Four different inhibitors (PARPi) are currently in the clinic for …
the DNA damage response. Four different inhibitors (PARPi) are currently in the clinic for …
Characterization of Medusavirus encoded histones reveals nucleosome-like structures and a unique linker histone
CM Toner, NM Hoitsma, S Weerawarana… - Nature …, 2024 - nature.com
The organization of DNA into nucleosomes is a ubiquitous and ancestral feature that was
once thought to be exclusive to the eukaryotic domain of life. Intriguingly, several …
once thought to be exclusive to the eukaryotic domain of life. Intriguingly, several …
HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase
Poly (ADP-ribose) polymerase 1 (PARP1) is an important player in the response to DNA
damage. Recently, Histone PARylation Factor (HPF1) was shown to be a critical modulator …
damage. Recently, Histone PARylation Factor (HPF1) was shown to be a critical modulator …
DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication
Nucleosome assembly in the wake of DNA replication is a key process that regulates cell
identity and survival. Chromatin assembly factor 1 (CAF-1) is a H3-H4 histone chaperone …
identity and survival. Chromatin assembly factor 1 (CAF-1) is a H3-H4 histone chaperone …
Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome
The chromatin remodeler ALC1 is recruited to and activated by DNA damage-induced poly
(ADP-ribose)(PAR) chains deposited by PARP1/PARP2/HPF1 upon detection of DNA …
(ADP-ribose)(PAR) chains deposited by PARP1/PARP2/HPF1 upon detection of DNA …