Genome editing took a dramatic turn with the development of the clustered regularly
interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) system …

The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …

The assembly of DNA from small fragments into large constructs has seen significant recent
development, becoming a pivotal technology in the ability to implement the vision of …

The CRISPR/Cas system, an innovative gene-editing tool, is emerging as a promising
technique for genome modifications. This straightforward technique was created based on …

Buried within the genomes of many microorganisms are genetic elements that encode rare-
cutting homing endonucleases that assist in the mobility of the elements that encode them …

Techniques for assembly of designed DNA sequences are important for synthetic biology.
So far, a few methods have been developed towards high-throughput seamless DNA …

Targeted genome engineering requires nucleases that introduce a highly specific double-
strand break in the genome that is either processed by homology-directed repair in the …

Embedding middle-scale artificial gene networks in live mammalian cells is one of the most
important future goals for cell engineering. However, the applications of the highly …

Background Tagging proteins is a standard method facilitating protein detection, purification
or targeting. When tagging a certain protein of interest, it is challenging to predict which tag …

The BioBricks standard has made the construction of DNA modules easier, quicker and
cheaper. So far, over 100 BioBricks assembly schemes have been developed and many of …