CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated
enzyme (Cas) is a naturally occurring genome editing tool adopted from the prokaryotic …

Therapeutic applications of nuclease-based genome editing would benefit from improved
methods for transgene integration via homology-directed repair (HDR). To improve HDR …

High-throughput functional characterization of genetic variants in their endogenous locus
has so far been possible only with methods that rely on homology-directed repair, which are …

The powerful CRISPR genome editing system is hindered by its off-target effects, and
existing computational tools achieved limited performance in genome-wide off-target …

Summary Interferon-γ (IFN-γ) signaling mediates host responses to infection, inflammation
and anti-tumor immunity. Mutations in the IFN-γ signaling pathway cause immunological …

CRISPR/Cas9-mediated cleavage of DNA, which depends on the endonuclease activity of
Cas9, has been widely used for gene editing due to its excellent programmability and …

Accelerated development of novel CRISPR/Cas9-based genome editing techniques
provides a feasible approach to introduce a variety of precise modifications in the …

After many years of painstaking research, the potential of gene therapy to cure severe β-
thalassemia is now becoming evident through recent clinical trials. Therapeutic protocols …

Microalgae as the photosynthetic organisms offer enormous promise in a variety of
industries, such as the generation of high-value byproducts, biofuels, pharmaceuticals …