Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
EJ Thak, SJ Yoo, HY Moon, HA Kang - FEMS yeast research, 2020 - academic.oup.com
Yeasts are prominent hosts for the production of recombinant proteins from industrial
enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways …
enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways …
Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Pichia pastoris is the most widely used microorganism for the production of secreted
industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a …
industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a …
An improved secretion signal enhances the secretion of model proteins from Pichia pastoris
Background Proteins can be secreted from a host organism with the aid of N-terminal
secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed …
secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed …
Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris
Y Ito, M Ishigami, N Hashiba, Y Nakamura… - Microbial …, 2022 - Wiley Online Library
In our previous study, we serendipitously discovered that protein secretion in the
methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor …
methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor …
An intron-derived motif strongly increases gene expression from transcribed sequences through a splicing independent mechanism in Arabidopsis thaliana
Certain introns significantly increase mRNA accumulation by a poorly understood
mechanism. These introns have no effect when located upstream, or more than~ 1 Kb …
mechanism. These introns have no effect when located upstream, or more than~ 1 Kb …
[HTML][HTML] Engineering Saccharomyces cerevisiae for application in integrated bioprocessing biorefineries
LS Minnaar, F Kruger, J Fortuin, LJ Hoffmeester… - Current Opinion in …, 2024 - Elsevier
Highlights•Consolidated bioprocessing conversion of cellulose to ethanol is not yet
viable.•The low value of energy products has shifted the focus to higher-value commodities.• …
viable.•The low value of energy products has shifted the focus to higher-value commodities.• …
Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia …
Y Huang, T Lin, L Lu, F Cai, J Lin, Y Jiang, Y Lin - Microbial Cell Factories, 2021 - Springer
Background Codon optimization is a common method to improve protein expression levels
in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to …
in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to …
A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris
H He, S Wu, M Mei, J Ning, C Li, L Ma… - … in Bioengineering and …, 2020 - frontiersin.org
Human lysozyme (hLYZ), known for its bacteriolytic activity, is widely applied in the food and
pharmaceutical industries as an antimicrobial agent. However, its extensive application was …
pharmaceutical industries as an antimicrobial agent. However, its extensive application was …
Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia …
Pichia pastoris (Komagataella spp.) has become one of the most important host organisms
for production of heterologous proteins of biotechnological interest, many of them …
for production of heterologous proteins of biotechnological interest, many of them …
Alternative secretory signal sequences for recombinant protein production in Pichia pastoris
M Karaoğlan - Enzyme and Microbial Technology, 2023 - Elsevier
Extracellular protein production is primarily preferred to facilitate the downstream processes
in recombinant protein production. Secretion of recombinant proteins is mediated by the …
in recombinant protein production. Secretion of recombinant proteins is mediated by the …