Synthetic protein scaffolds for the colocalisation of co-acting enzymes
J Vanderstraeten, Y Briers - Biotechnology Advances, 2020 - Elsevier
Nature relies on complexes of colocated enzymes to efficiently perform multiple catalytic
steps. Such enzyme colocalisation promotes substrate channelling, enhances the activity of …
steps. Such enzyme colocalisation promotes substrate channelling, enhances the activity of …
Natural and designer cellulosomes: A potential tool for enhancing microbial additive-mediated lignocellulosic agricultural waste composting
Lignocellulosic agricultural waste composting is a cost-effective approach for remedying
such waste. The complex, recalcitrant nature of lignocellulosic agricultural waste hinders its …
such waste. The complex, recalcitrant nature of lignocellulosic agricultural waste hinders its …
A seawater-based open and continuous process for polyhydroxyalkanoates production by recombinant Halomonas campaniensis LS21 grown in mixed substrates
Background High-cost production of bioplastics polyhydroxyalkanoates (PHA) is a major
concern for their large scale application. In order to produce PHA economically, new …
concern for their large scale application. In order to produce PHA economically, new …
Designed protein cages as scaffolds for building multienzyme materials
The functions of enzymes can be strongly affected by their higher-order spatial
arrangements. In this study we combine multiple new technologies—designer protein cages …
arrangements. In this study we combine multiple new technologies—designer protein cages …
Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome
Efficient breakdown of lignocellulose polymers into simple molecules is a key technological
bottleneck limiting the production of plant-derived biofuels and chemicals. In nature, plant …
bottleneck limiting the production of plant-derived biofuels and chemicals. In nature, plant …
A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates
Y Vazana, Y Barak, T Unger, Y Peleg… - Biotechnology for …, 2013 - Springer
Background Select cellulolytic bacteria produce multi-enzymatic cellulosome complexes that
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …
Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability
Background The concerted action of three complementary cellulases from Clostridium
thermocellum, engineered to be stable at elevated temperatures, was examined on a …
thermocellum, engineered to be stable at elevated temperatures, was examined on a …
Creation of a functional hyperthermostable designer cellulosome
Background Renewable energy has become a field of high interest over the past decade,
and production of biofuels from cellulosic substrates has a particularly high potential as an …
and production of biofuels from cellulosic substrates has a particularly high potential as an …
[HTML][HTML] Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction
Production of economically viable bioethanol is potentially an environmentally and
financially worthwhile endeavor. One major source for fermentable sugars is lignocellulose …
financially worthwhile endeavor. One major source for fermentable sugars is lignocellulose …
Continually emerging mechanistic complexity of the multi-enzyme cellulosome complex
SP Smith, EA Bayer, M Czjzek - Current Opinion in Structural Biology, 2017 - Elsevier
Highlights•Cellulosome complexes mediate efficient hydrolysis of plant-derived
polysaccharides.•Computational biology reveals structure-function relationships of …
polysaccharides.•Computational biology reveals structure-function relationships of …