Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …

Transcription is episodic, consisting of a series of discontinuous bursts. Using live-imaging
methods and quantitative analysis, we examine transcriptional bursting in living Drosophila …

BACKGROUND The eukaryotic genome is hierarchically organized in the cell nucleus into
DNA loops, chromatin domains, compartments, and, ultimately, chromosomes. Many of the …

In prokaryotes and eukaryotes, most genes appear to be transcribed during short periods
called transcriptional bursts, interspersed by silent intervals. We describe how such bursts …

In individual cells, transcription is a random process obeying single-molecule kinetics. Often,
it occurs in a bursty, intermittent manner. The frequency and size of these bursts affect the …

Gene expression occurs either as an episodic process, characterized by pulsatile bursts, or
as a constitutive process, characterized by a Poisson-like accumulation of gene products. It …

Elongation is becoming increasingly recognized as a critical step in eukaryotic
transcriptional regulation. Although traditional genetic and biochemical studies have …

Spatiotemporal patterns of gene expression are fundamental to every developmental
program. The resulting macroscopic domains have been mainly characterized by their levels …

Gene activity is described by the time series of discrete, stochastic mRNA production events.
This transcriptional time series shows intermittent, bursty behavior. One consequence of this …