Extended mechanism of the plasminogen activator staphylokinase revealed by global kinetic analysis: 1000-fold higher catalytic activity than That of clinically used …

M Toul, D Nikitin, M Marek, J Damborsky… - ACS Catalysis, 2022 - ACS Publications
The plasminogen activator staphylokinase is a fibrin-specific thrombolytic biomolecule and
an attractive target for the development of effective myocardial infarction and stroke therapy …

C-terminal lysine residues enhance plasminogen activation by inducing conformational flexibility and stabilization of activator complex of staphylokinase with plasmin

P Kaur, D Sethi, MD Hade, J Kaur, KL Dikshit - Archives of Biochemistry …, 2023 - Elsevier
Staphylokinase (SAK), a potent fibrin-specific plasminogen activator secreted by
Staphylococcus aureus, carries a pair of lysine at the carboxy-terminus that play a key role in …

High-level expression of non-glycosylated and active staphylokinase from Pichia pastoris

A Apte-Deshpnade, G Mandal, S Soorapaneni… - Biotechnology …, 2009 - Springer
Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders.
Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris …

[PDF][PDF] Isolation, cloning and expression of mature staphylokinase from lysogenic Staphylococcus aureus collected from a local wound sample in a salt inducible E. coli …

KK Pulicherla, GS Gadupudi, VPB Rekha… - International Journal of …, 2011 - Citeseer
Staphylokinase, a 163 amino aci d bacterial protein from Staphylococcus aureus is reported
to have a therapeutic function and used as a potential alternative for the available clot …

Skizzle is a novel plasminogen-and plasmin-binding protein from Streptococcus agalactiae that targets proteins of human fibrinolysis to promote plasmin generation

KG Wiles, P Panizzi, HK Kroh, PE Bock - Journal of Biological Chemistry, 2010 - ASBMB
Skizzle (SkzL), secreted by Streptococcus agalactiae, has moderate sequence identity to
streptokinase and staphylokinase, bacterial activators of human plasminogen (Pg). SkzL …

Support vector machine (SVM) based multiclass prediction with basic statistical analysis of plasminogen activators

S Muthukrishnan, M Puri, C Lefevre - BMC research notes, 2014 - Springer
Abstract Background Plasminogen (Pg), the precursor of the proteolytic and fibrinolytic
enzyme of blood, is converted to the active enzyme plasmin (Pm) by different plasminogen …

High yielding recombinant Staphylokinase in bacterial expression system—cloning, expression, purification and activity studies

N Mandi, S Soorapaneni, S Rewanwar, P Kotwal… - Protein expression and …, 2009 - Elsevier
Staphylokinase (SAK) is emerging as an important thrombolytic agent. In this report, we
describe the cloning, expression, purification and activity studies of the SAK gene of …

Identification of a new exosite involved in catalytic turnover by the streptokinase-plasmin activator complex during human plasminogen activation

R Aneja, M Datt, B Singh, S Kumar, G Sahni - Journal of biological …, 2009 - ASBMB
With the goal of identifying hitherto unknown surface exosites of streptokinase involved in
substrate human plasminogen recognition and catalytic turnover, synthetic peptides …

[HTML][HTML] Enhanced plasminogen activation by staphylokinase in the presence of streptokinase β/βγ domains: plasminogen kringles play a role

M Dahiya, G Rajamohan, KL Dikshit - FEBS letters, 2005 - Elsevier
Presence of isolated β or βγ domains of streptokinase (SK) increased the catalytic activity of
staphylokinase (SAK)–plasmin (Pm) complex up to 60%. In contrast, fusion of SK β or βγ …

Integration of VEK‐30 peptide enhances fibrinolytic properties of staphylokinase

T Bhando, S Singh, MD Hade, J Kaur… - Biotechnology and …, 2021 - Wiley Online Library
Abstract Staphylokinase (SAK), a 136 amino acid bacterial protein with profibrinolytic
properties, has emerged as an important thrombolytic agent because of its fibrin specificity …