Latest approaches for efficient protein production in drug discovery
Introduction: Pharmaceutical research looks to discover and develop new compounds which
influence the function of disease-associated proteins or respective protein–protein …
influence the function of disease-associated proteins or respective protein–protein …
A novel gyrase inhibitor from toxin–antitoxin system expressed by Staphylococcus aureus
Toxin–antitoxin (TA) systems consist of a toxin inhibiting essential cellular functions (such as
DNA, RNA and protein synthesis), and its cognate antitoxin neutralizing the toxicity …
DNA, RNA and protein synthesis), and its cognate antitoxin neutralizing the toxicity …
Recent applications of isotopic labeling for protein NMR in drug discovery
H Hiroaki - Expert Opinion on Drug Discovery, 2013 - Taylor & Francis
Introduction: Nuclear magnetic resonance (NMR) applications in drug discovery are
classified into two categories: ligand-based methods and protein-based methods. The latter …
classified into two categories: ligand-based methods and protein-based methods. The latter …
Heme interaction of the intrinsically disordered N-terminal peptide segment of human cystathionine-β-synthase
Abstract Cystathionine-β-synthase (CBS) belongs to a large family of pyridoxal 5'-phosphate
(PLP)-dependent enzymes, responsible for the sulfur metabolism. The heme-dependent …
(PLP)-dependent enzymes, responsible for the sulfur metabolism. The heme-dependent …
Engineering soluble tobacco etch virus protease accompanies the loss of stability
J Fang, L Chen, B Cheng, J Fan - Protein Expression and Purification, 2013 - Elsevier
Tobacco etch virus protease (TEVp) is a widely used tool enzyme in biological studies. To
improve the solubility of recombinant TEVp, three variants, including the double mutant …
improve the solubility of recombinant TEVp, three variants, including the double mutant …
An endogenous protein inhibitor, YjhX (TopAI), for topoisomerase I from Escherichia coli
Almost all free-living bacteria contain toxin-antitoxin (TA) systems on their genomes and the
targets of toxins are highly diverse. Here, we found a novel, previously unidentified TA …
targets of toxins are highly diverse. Here, we found a novel, previously unidentified TA …
Characterization of YjjJ toxin of Escherichia coli
Reminiscent of eukaryotic apoptotic programmed cell death, bacteria also contain a large
number of suicide genes, which are in general co-expressed with their cognate antitoxin …
number of suicide genes, which are in general co-expressed with their cognate antitoxin …
Semi-synthesis of labeled proteins for spectroscopic applications
L De Rosa, A Russomanno, A Romanelli, LD D'Andrea - Molecules, 2013 - mdpi.com
Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered
the possibility of preparing large proteins. The introduction of native chemical ligation in the …
the possibility of preparing large proteins. The introduction of native chemical ligation in the …
Segmental isotopic labeling of proteins for NMR study using intein technology
Segmental isotopic labeling of samples for NMR studies is attractive for large complex
biomacromolecular systems, especially for studies of function-related protein–ligand …
biomacromolecular systems, especially for studies of function-related protein–ligand …
Engineered solubility tag for solution NMR of proteins
AM Ruschak, JD Rose, MP Coughlin… - Protein …, 2013 - Wiley Online Library
The low solubility of many proteins hinders large scale expression and purification as well
as biophysical measurements. Here, we devised a general strategy to solubilize a protein by …
as biophysical measurements. Here, we devised a general strategy to solubilize a protein by …