Metabolic diversity in microorganisms can provide the basis for creating novel biochemical
products. However, most metabolic engineering projects utilize a handful of established …

Promoter engineering is an enabling technology in metabolic engineering and synthetic
biology. As an indispensable part of synthetic biology, the promoter is a key factor in …

Precise control of gene expression is essential for flux redistribution in metabolic pathways.
Although the CRISPR interference (CRISPRi) system can effectively repress gene …

Promoters are one of the most critical regulatory elements controlling metabolic pathways.
However, the fast and accurate prediction of promoter strength remains challenging, leading …

Microbial consortia have been considered potential platforms for bioprocessing applications.
However, the complexity in process control owing to the use of multiple strains necessitates …

2′-Fucosyllactose (2′-FL) is the most abundant oligosaccharide in human milk. In this
study, a highly efficient biosynthetic route for 2′-FL production was designed via the de …

The antimicrobial resistance crisis along with challenges of antimicrobial discovery revealed
the vital necessity to develop new antibiotics. Many of the animal proline-rich antimicrobial …

Methyluridine (5-MU) is a prominent intermediate for industrial synthesis of several antiviral-
drugs, however, its availability over the past decades has overwhelmingly relied on …

Methane is an important target for reducing greenhouse gases emission, owing to its
abundance and highly global warming potential. Bioconversion of methane to value-added …

Despite industrial bio-manufacturing progress using Bacillus licheniformis, the absence of a
well-characterized toolbox allowing precise regulation of multiple genes limits its expansion …