Scanning probe microscopy (SPM), a key invention in nanoscience, has by now been
extended to a wide spectrum of basic and applied fields. Its application to basic science led …

TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …

CRISPR-Cas9 genome editing has enabled advanced T cell therapies, but occasional loss
of the targeted chromosome remains a safety concern. To investigate whether Cas9-induced …

Understanding structural dynamics of biomolecules at the single-molecule level is vital to
advancing our knowledge of molecular mechanisms. Currently, there are few techniques …

The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects.
Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher …

CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …

ChRmine, a recently discovered pump-like cation-conducting channelrhodopsin, exhibits
puzzling properties (large photocurrents, red-shifted spectrum, and extreme light sensitivity) …

The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little is
known about the kinetics and fidelity of the double-strand break (DSB) repair process that …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Cas9 and Cas12a are multidomain CRISPR‐associated nucleases that can be programmed
with a guide RNA to bind and cleave complementary DNA targets. The guide RNA …