The autophagic engulfment of cytoplasm and organelles and their delivery to the vacuole
have long been speculated to play an essential role in bulk protein turnover in plants. Until …

▪ Abstract Plant membrane trafficking shares many features with other eukaryotic organisms,
including the machinery for vesicle formation and fusion. However, the plant endomembrane …

The vacuole/lysosome serves an important recycling function during starvation and
senescence in eukaryotes via a process called autophagy. Here bulk cytosolic constituents …

Vacuolar compartments associated with leaf senescence and the subcellular localization of
the senescence‐specific cysteine‐protease SAG12 (senescence‐associated gene 12) were …

The pH homeostasis of endomembranes is essential for cellular functions. In order to
provide direct pH measurements in the endomembrane system lumen, we targeted …

The mechanisms that control protein transport between the endoplasmic reticulum (ER) and
the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves …

Plants produce a very large number of specialized compounds that must be transported from
their site of synthesis to the sites of storage or disposal. Anthocyanin accumulation has …

For noninvasive in vivo measurements of intra-and extracellular ion concentrations, we
produced transgenic Arabidopsis expressing pH and calcium indicators in the cytoplasm …

Green fluorescent protein (GFP) makes it possible for organelles and protein transport
pathways to be visualized in living cells. However, GFP fluorescence has not yet been …

We generated fusions between three Arabidopsis (Arabidopsis thaliana) tonoplast intrinsic
proteins (TIPs; α-, γ-, and δ-TIP) and yellow fluorescent protein (YFP). We also produced …