STED microscopy and its applications: new insights into cellular processes on the nanoscale

T Müller, C Schumann, A Kraegeloh - ChemPhysChem, 2012 - Wiley Online Library
For about a decade, superresolution fluorescence microscopy has been advancing steadily,
maturing from the proof‐of‐principle stage to routine application. Of the various techniques …

Nanoparticles for super-resolution microscopy: intracellular delivery and molecular targeting

SK Pramanik, S Sreedharan, R Tiwari, S Dutta… - Chemical Society …, 2022 - pubs.rsc.org
Following an overview of the approaches and techniques used to acheive super-resolution
microscopy, this review presents the advantages supplied by nanoparticle based probes for …

AIE nanoparticles with high stimulated emission depletion efficiency and photobleaching resistance for long‐term super‐resolution bioimaging

D Li, W Qin, B Xu, J Qian, BZ Tang - Advanced Materials, 2017 - Wiley Online Library
Stimulated emission depletion (STED) nanoscopy is a typical super‐resolution imaging
technique that has become a powerful tool for visualizing intracellular structures on the …

Effects of polarization on the de-excitation dark focal spot in STED microscopy

X Hao, C Kuang, T Wang, X Liu - Journal of Optics, 2010 - iopscience.iop.org
The size of the dark focal spot directly determines the resolution and stability of stimulated
emission depletion (STED) microscopy. This paper investigates the relationship between the …

Recent advances in STED and RESOLFT super-resolution imaging techniques

R Sharma, M Singh, R Sharma - … Acta Part A: Molecular and Biomolecular …, 2020 - Elsevier
Stimulated emission depletion (STED) and reversible saturable optical fluorescence
transition (RESOLFT) microscopy are the super-resolution imaging techniques that can …

Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy

J Yu, X Sun, F Cai, Z Zhu, A Qin, J Qian, B Tang… - Optics Letters, 2015 - opg.optica.org
We present a preliminary study which explores the potential of aggregation-induced
emission (AIE) luminogen as a new fluorescent probe for STED microscopy. Compared with …

Tuning donut profile for spatial resolution in stimulated emission depletion microscopy

B Neupane, F Chen, W Sun, DT Chiu… - Review of Scientific …, 2013 - pubs.aip.org
In stimulated emission depletion (STED)-based or up-conversion depletion-based super-
resolution optical microscopy, the donut-shaped depletion beam profile is of critical …

Fluorescence nanoscopy. Methods and applications

J Requejo-Isidro - Journal of chemical biology, 2013 - Springer
Fluorescence nanoscopy refers to the experimental techniques and analytical methods used
for fluorescence imaging at a resolution higher than conventional, diffraction-limited …

Review of recent developments in stimulated emission depletion microscopy: applications on cell imaging

B Neupane, FS Ligler, G Wang - Journal of biomedical optics, 2014 - spiedigitallibrary.org
Stimulated emission depletion (STED) microscopy is one type of far-field optical technique
demonstrated to provide subdiffraction resolution. STED microscopy utilizes a donut-shaped …

Organic nanoparticles with ultrahigh stimulated emission depletion efficiency for low-power STED nanoscopy

Z Man, Z Lv, Z Xu, H Cui, Q Liao, L Zheng, X **, Q He… - Nanoscale, 2019 - pubs.rsc.org
Stimulated emission depletion (STED) nanoscopy is a powerful sub-diffraction imaging tool
to probe subcellular structures and organelles. Conventional organic dyes require high …