Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of
molecular dynamics, and it has been widely applied in diverse fields, eg, biomedicine …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation
process minimizes fluorophore bleaching and phototoxic effects. Because biological …

Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure
concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to …

The graded distribution of morphogens underlies many of the tissue patterns that form
during development. How morphogens disperse from a localized source and how gradients …

Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …

For a little more than a century, fluorescence microscopy has been an essential source of
major discoveries in cell biology. Recent developments improved both visualization and …

This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …

Understanding of cellular regulatory pathways that involve lipid membranes requires the
detailed knowledge of their physical state and structure. However, map** the viscosity and …