As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

The diagnosis of infectious diseases is ineffective when the diagnostic test does not meet
one or more of the necessary standards of affordability, accessibility, and accuracy. The …

Healthcare-associated infections (HAIs) are the most common adverse events in healthcare
and constitute a major global public health concern. Surveillance represents the foundation …

Real-time polymerase chain reaction (qPCR) enables accurate detection and quantification
of nucleic acids and has become a fundamental tool in biological sciences, bioengineering …

Despite the advantages of digital nucleic acid analysis (DNAA) in terms of sensitivity,
precision, and resolution, current DNAA methods commonly suffer a limitation in …

Intensive care units (ICUs) are specialized environments dedicated to the management of
critically ill patients, who are particularly susceptible to drug-resistant bacteria. Among these …

Introduction Machine learning (ML) is increasingly being used to predict antimicrobial
resistance (AMR). This review aims to provide physicians with an overview of the literature …

Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in
molecular biology and diagnostics, offering exceptional precision and sensitivity in nucleic …

Background Food authenticity is essential to protect against fraudulent activities and ensure
food safety. However, traditional authentication methods have limited capabilities due to …

Abstract β-Lactamases are bacterial enzymes that inactivate β-lactam antibiotics and, as
such, are the most prevalent cause of antibiotic resistance in Gram-negative bacteria. The …