Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

Manipulation of individual molecules with optical tweezers provides a powerful means of
interrogating the structure and folding of proteins. Mechanical force is not only a relevant …

Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces
and motions associated with biological molecules and enzymatic activity. The most common …

We present a new estimator for computing free energy differences and thermodynamic
expectations as well as their uncertainties from samples obtained from multiple equilibrium …

Single-molecule magnetic tweezers deliver magnetic force and torque to single target
molecules, permitting the study of dynamic changes in biomolecular structures and their …

It has been over 20 years since the pioneering work of Arthur Ashkin, and in the intervening
years, the field of optical tweezers has grown tremendously. Optical tweezers are now being …

Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Optical tweezers (OT) have emerged as an essential tool for manipulating single biological
cells and performing sophisticated biophysical/biomechanical characterizations. Distinct …