Since the advent of the green fluorescent protein, the subcellular localization, mobility,
transport routes and binding interactions of proteins can be studied in living cells. Live cell …

The ability to visualize, track, and quantify molecules and events in living cells with high
spatial and temporal resolution is essential for understanding biological systems. Only …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

The mathematical concept of fluorescence correlation spectroscopy (FCS) 1 (1) emerged
from quasi-elastic light scattering (QELS) spectroscopy (2) in the early 1970s. Compared to …

The fluid mosaic membrane model proved to be a very useful hypothesis in explaining
many, but certainly not all, phenomena taking place in biological membranes. New …

Understanding biology at the single-cell level requires simultaneous measurements of
biochemical parameters and behavioral characteristics in individual cells. Here, the output of …

Fluorescence correlation spectroscopy (FCS) can provide a wealth of information about
biological and chemical systems on a broad range of time scales (< 1μs to> 1s). Numerical …

Fluorescence correlation spectroscopy (FCS) is a time-averaging fluctuation analysis of
small molecular ensembles, combining maximum sensitivity with high statistical confidence …

Fluorescence proteins (FPs) have been widely used for live-cell imaging in the past decade.
This review summarizes the recent advances in FP development and imaging technologies …

Nature has evolved an optimal synthetic factory in the form of translational and
posttranslational processes by which millions of proteins with defined primary sequences …