[HTML][HTML] The CRISPR-Cas toolbox and gene editing technologies

G Liu, Q Lin, S **, C Gao - Molecular cell, 2022 - cell.com
The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Structural biology of CRISPR–Cas immunity and genome editing enzymes

JY Wang, P Pausch, JA Doudna - Nature Reviews Microbiology, 2022 - nature.com
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …

The next generation of CRISPR–Cas technologies and applications

A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering

H Altae-Tran, S Kannan, AJ Suberski, KS Mears… - Science, 2023 - science.org
Microbial systems underpin many biotechnologies, including CRISPR, but the exponential
growth of sequence databases makes it difficult to find previously unidentified systems. In …

A time-resolved, multi-symbol molecular recorder via sequential genome editing

J Choi, W Chen, A Minkina, FM Chardon, CC Suiter… - Nature, 2022 - nature.com
DNA is naturally well suited to serve as a digital medium for in vivo molecular recording.
However, contemporary DNA-based memory devices are constrained in terms of the …

The biology of CRISPR-Cas: backward and forward

F Hille, H Richter, SP Wong, M Bratovič, S Ressel… - Cell, 2018 - cell.com
In bacteria and archaea, clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …

Molecular digital data storage using DNA

L Ceze, J Nivala, K Strauss - Nature Reviews Genetics, 2019 - nature.com
Molecular data storage is an attractive alternative for dense and durable information storage,
which is sorely needed to deal with the growing gap between information production and the …

DNA sequencing at 40: past, present and future

J Shendure, S Balasubramanian, GM Church… - Nature, 2017 - nature.com
This review commemorates the 40th anniversary of DNA sequencing, a period in which we
have already witnessed multiple technological revolutions and a growth in scale from a few …

Applications of CRISPR technologies in research and beyond

R Barrangou, JA Doudna - Nature biotechnology, 2016 - nature.com
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

Noninvasive assessment of gut function using transcriptional recording sentinel cells

F Schmidt, J Zimmermann, T Tanna, R Farouni… - Science, 2022 - science.org
Transcriptional recording by CRISPR spacer acquisition from RNA endows engineered
Escherichia coli with synthetic memory, which through Record-seq reveals transcriptome …