Optical clearing methods: an overview of the techniques used for the imaging of 3D spheroids

EC Costa, DN Silva, AF Moreira… - Biotechnology and …, 2019 - Wiley Online Library
Spheroids have emerged as in vitro models that reproduce in a great extent the architectural
microenvironment found in human tissues. However, the imaging of 3D cell cultures is highly …

Nanosecond pulsed electric field (nsPEF): Opening the biotechnological Pandora's box

AR Ruiz-Fernández, L Campos… - International Journal of …, 2022 - mdpi.com
Nanosecond Pulsed Electric Field (nsPEF) is an electrostimulation technique first developed
in 1995; nsPEF requires the delivery of a series of pulses of high electric fields in the order of …

Pathways of membrane solubilization: A structural study of model lipid vesicles exposed to classical detergents

VA Bjørnestad, R Lund - Langmuir, 2023 - ACS Publications
Understanding the pathways of solubilization of lipid membranes is of high importance for
their use in biotechnology and industrial applications. Although lipid vesicle solubilization by …

Elastic cationic liposomes for vitamin C delivery: Development, characterization and skin absorption study

AC Caritá, JR de Azevedo, Y Chevalier… - International Journal of …, 2023 - Elsevier
The influence of hydrophilic surfactants acting on the membrane elasticity of liposomes on
the skin absorption of vitamin C is investigated. The purpose of encapsulation inside cationic …

Galactose to tagatose isomerization at moderate temperatures with high conversion and productivity

JR Bober, NU Nair - Nature Communications, 2019 - nature.com
There are many industrially-relevant enzymes that while active, are severely limited by
thermodynamic, kinetic, or stability issues (isomerases, lyases, transglycosidases). In this …

[HTML][HTML] Beyond the standard model of solubilization: Non-ionic surfactants induce collapse of lipid vesicles into rippled bilamellar nanodiscs

VA Bjørnestad, F Soto-Bustamante, G Tria… - Journal of Colloid and …, 2023 - Elsevier
Hypothesis Although solubilization of lipid membranes has been studied extensively,
questions remain regarding the structural pathways and metastable structures involved. This …