Assessment of bisphosphonate treated-osteoblast behaviors by conventional assays and a simple digital image analysis

S Patntirapong, C Chanruangvanit, K Lavanrattanakul… - Acta histochemica, 2021 - Elsevier
The main objective of this study was to analyze the changes in osteoblast behaviors by two
different methods, a simple digital image analysis method and conventional assays …

Authentication, characterization and contamination detection of cell lines, xenografts and organoids by barcode deep NGS sequencing

X Chen, W Qian, Z Song, QX Li… - NAR Genomics and …, 2020 - academic.oup.com
Misidentification and contamination of biobank samples (eg cell lines) have plagued
biomedical research. Short tandem repeat (STR) and single-nucleotide polymorphism …

MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9

H Zhang, X Lu, Y Hao, L Tang, Z He - Acta histochemica, 2020 - Elsevier
Emerging evidence has unraveled the important implications of microRNAs (miRNAs/miRs)
in intracerebral hemorrhage (ICH). The aim of the present study was to assess the possible …

[HTML][HTML] Development and Evaluation of a New qPCR Assay for the Detection of Mycoplasma in Cell Cultures

JA Carrillo-Ávila, A de la Fuente… - Current Issues in …, 2023 - mdpi.com
In recent years, cell culture has become an important tool not only in research laboratories,
but also in diagnostic and biotechnological development laboratories. Mycoplasma …

Performance of PCR-based and Bioluminescent assays for mycoplasma detection

P Falagan-Lotsch, TS Lopes, N Ferreira… - Journal of …, 2015 - Elsevier
Contaminated eukaryotic cell cultures are frequently responsible for unreliable results.
Regulatory entities request that cell cultures must be mycoplasma-free. Mycoplasma …

Inhibition of macrophage viability by bound and free bisphosphonates

S Patntirapong, P Phupunporn, D Vanichtantiphong… - Acta …, 2019 - Elsevier
Introduction Long-term administration of bisphosphonates (BPs) may cause osteonecrosis of
the jaw (BRONJ). After administration, 50% of BPs in the circulation rapidly binds to calcium …

Establishment and characterization of two novel cholangiocarcinoma cell lines

Y Zhang, J Luo, X Dong, F Yang, M Zhang… - Annals of Surgical …, 2019 - Springer
Background Cholangiocarcinoma (CCA) is a rare, aggressive and highly lethal tumor. The
disease is very difficult to diagnose and multi-modality treatments are ineffective. To improve …

Tumor elastography and its association with cell-free tumor DNA in the plasma of breast tumor patients: a pilot study

Y Hao, W Yang, W Zheng, X Chen… - … Imaging in Medicine …, 2021 - pmc.ncbi.nlm.nih.gov
Background Breast tumor stiffness, which can be objectively and noninvasively evaluated by
ultrasound elastography (UE), has been useful for the differentiation of benign and …

[HTML][HTML] Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures

J Krzysztoń-Russjan, J Chudziak, M Bednarek… - Diagnostics, 2021 - mdpi.com
Mycoplasma, Acholeplasma, and Ureaplasma sp. are atypical bacteria responsible for in
vitro cell culture contaminations that can warp the results. These bacteria also cause human …

Detection of mycoplasma contamination of cell culture by a loop-mediated isothermal amplification method

Z Soheily, M Soleimani, MA Keivan - Cell Journal (Yakhteh), 2018 - pmc.ncbi.nlm.nih.gov
Objective Mycoplasmas are major contaminants of cell culture and affect in vitro biological
and diagnostic tests. Mycoplasma detection is conducted using culture and molecular …