Complete and accurate DNA replication requires the progression of replication forks through
DNA damage, actively transcribed regions, structured DNA and compact chromatin. Recent …

High-fidelity DNA replication is critical for the faithful transmission of genetic information to
daughter cells. Following genotoxic stress, specialized DNA damage tolerance pathways …

Mutations in BRCA1 or BRCA2 (BRCA) is synthetic lethal with poly (ADP-ribose)
polymerase inhibitors (PARPi). Lethality is thought to derive from DNA double-stranded …

Using CRISPR-Cas9 nicking enzymes, we examined the interaction between the replication
machinery and single-strand breaks, one of the most common forms of endogenous DNA …

PRIMPOL repriming allows DNA replication to skip DNA lesions, leading to ssDNA gaps.
These gaps must be filled to preserve genome stability. Using a DNA fiber approach to …

BRCA1/2 mutant tumor cells display an elevated mutation burden, the etiology of which
remains unclear. Here, we report that these cells accumulate ssDNA gaps and spontaneous …

Replication fork reversal safeguards genome integrity as a replication stress response. DNA
translocases and the RAD51 recombinase catalyze reversal. However, it remains unknown …

Tumor suppressor BRCA2 functions in homology-directed repair (HDR), the protection of
stalled replication forks, and the suppression of replicative gaps, but their relative …

Chromatin is dynamically reorganized when DNA replication forks are challenged. However,
the process of epigenetic reorganization and its implication for fork stability is poorly …

DNA replication stress can stall replication forks, leading to genome instability. DNA damage
tolerance pathways assist fork progression, promoting replication fork reversal, translesion …