The yeast Saccharomyces cerevisiae has been a favorite organism for pioneering studies
on nutrient-sensing and signaling mechanisms. Many specific nutrient responses have been …

Although the roots of Ras sprouted from the rich history of retrovirus research, it was the
discovery of mutationally activated RAS genes in human cancer in 1982 that stimulated an …

GTPases are conserved molecular switches, built according to a common structural design.
Rapidly accruing knowledge of individual GTPases—crystal structures, biochemical …

Small GTP-binding proteins (G proteins) exist in eukaryotes from yeast to human and
constitute a superfamily consisting of more than 100 members. This superfamily is …

We developed a method for immunoaffinity purification of Saccharomyces cerevisiae
adenylyl cyclase based on creating a fusion with a small peptide epitope. Using …

Mammalian ras genes are thought to be critical in the regulation of cellular proliferation and
differentiation and are mutated in∼ 30% of all human tumors. However, N-ras and H-ras are …

This article reviews the origin and evolution of high throughput screening (HTS) through the
experience of an individual pharmaceutical company, revealing some of the mysteries of the …

Eukaryotic cells possess an exquisitely interwoven and fine-tuned series of signal
transduction mechanisms with which to sense and respond to the ubiquitous fermentable …

We have identified a new family of ras genes, the rho genes, which share several properties
with the more classical ras gene family consisting of N-, K-, and H-ras. The rho genes, first …

The Harvey murine sarcoma virus (Ha-MuSV) transforming gene, v-ras H, encodes a 21,000
molecular weight protein (p21) that is closely related to the p21 proteins encoded by the …