The genomes of bacteria contain fewer genes and substantially less noncoding DNA than
those of eukaryotes, and as a result, they have much less raw material to invent new traits …

Adaptive laboratory evolution (ALE) is a useful experimental methodology for fundamental
scientific research and industrial applications to create microbial cell factories. By using ALE …

Promiscuous enzymes often serve as the starting point for the evolution of novel functions.
Yet, the extent to which the promiscuity of an individual enzyme can be harnessed several …

Promiscuity is the coincidental ability of an enzyme to catalyze its native reaction and
additional reactions that are not biological functions in the same active site. Promiscuity …

Summary The polyamine putrescine (1, 4‐diaminobutane) contributes to cellular fitness in
most organisms, where it is derived from the amino acids ornithine or arginine. In the …

Engineering microbes to utilize non-conventional substrates could create short and efficient
pathways to convert substrate into product. In this study, we designed and constructed a two …

Microbial-based chemical synthesis serves as a promising approach for sustainable
production of industrially important products. However, limited production performance …

Ethanol, a promising nonfood carbon source from syngas fermentation, has recently been
used in the biosynthesis of bulk chemicals in industrial biomanufacturing. In this study, the …

Pyridoxal 5′‐phosphate (PLP) is an organic cofactor employed by~ 4% of enzymes. The
structure of the PLP cofactor allows for the stabilization of carbanions through resonance. A …

Acetyl-CoA is a key metabolic intermediate and the product of various natural and synthetic
one-carbon (C1) assimilation pathways. While an efficient conversion of acetyl-CoA into …